中国中医药信息杂志2025，Vol.32Issue(4)：32-41,10.DOI:10.19879/j.cnki.1005-5304.202408526
GEO数据库联合网络药理学及实验验证探讨薏竹温胆汤治疗湿疹作用机制
Exploration on the Mechanism of Yizhu Wendan Decoction in Treating Eczema Based on GEO Database Combined with Network Pharmacology and Experimental Verification
摘要
Abstract
Objective To explore the mechanism of Yizhu Wendan Decoction in treating eczema through GEO database combined with network pharmacology and experimental verification.Methods TCMSP,BATMAN-TCM and ETCM databases were used to screen the active components of Yizhu Wendan Decoction.Disease target information related to eczema was collected through GEO database.The drug-component-target network and PPI network were constructed by intersections of active component targets and disease targets.GO and KEGG pathway enrichment analyses were performed using DAVID database.CCK-8 method was used to screen out the optimal intervention concentration of freeze-dried powder of Yizhu Wendan Decoction.HaCaT cells were divided into control group,model group,Yizhu Wendan Decoction low concentration group,Yizhu Wendan Decoction high concentration group,si-IL-17RA group,si-IL-17RA+Yizhu Wendan Decoction low concentration group,si-IL-17RA+Yizhu Wendan Decoction high concentration group,Dexamethasone group,si-IL-17RA+Dexamethasone group.Each group was given relevant intervention.The expressions of chemokines and inflammatory factors were detected by qPCR.EdU and Annexin V-FITC/PI double staining were used to detect cell proliferation and apoptosis.Western blot was performed to detect the expressions of proteins related to apoptosis,skin barrier and IL-17 signaling pathway.Results By using databases,180 active components of Yizhu Wendan Decoction were obtained.Combined with GEO database microarrays related to eczema(GSE6012 and GSE57225),8 potential targets of Yizhu Wendan Decoction in the treatment of eczema were obtained.KEGG enrichment pathway mainly involved IL-17 signaling pathway,lipid and atherosclerotic,TNF signaling pathway,fluid shear stress and atherosclerotic,etc.When Yizhu Wendan Decoction freeze-dried powder concentration was 100 μg/mL,cell viability was the strongest.Yizhu Wendan Decoction could significantly inhibit the mRNA expressions of chemokines and inflammatory factors CCL17,CCL22,IL-1β,TNF-α,IL-6,IFN-γ,and increase the mRNA expression of IL-4 in eczema.It promoted the proliferation of HaCaT cells,increased the protein expression of Bcl-2,and reduced the protein expressions of Bad and Cleaved Caspase-3,thus inhibiting HaCaT cells apoptosis;promoted the protein expressions of FLG and LOR,and reduced the expression of MMP9,MMP1,CCL2,FOSL1,IL-17RA proteins in IL-17 signaling pathway.Conclusion Yizhu Wendan Decoction can treat eczema with multiple components,multiple pathways and multiple targets,promote the proliferation of HaCaT cells,inhibit their apoptosis,and restore the skin barrier.Its mechanism may be related to inhibiting the activation of IL-17 signaling pathway.关键词
湿疹/薏竹温胆汤/分消走泄/网络药理学/作用机制/细胞实验Key words
eczema/Yizhu Wendan Decoction/dissipate and drain/network pharmacology/mechanism/cell experiment分类
医药卫生引用本文复制引用
王钇杰,郭婷婷,李勇军,李孜怡,张萌,史梦迪,谷胜男,王有鹏,李志军..GEO数据库联合网络药理学及实验验证探讨薏竹温胆汤治疗湿疹作用机制[J].中国中医药信息杂志,2025,32(4):32-41,10.基金项目
黑龙江省中医药科研项目(GY2022-24) （GY2022-24）
黑龙江省博士后创新科研项目(LBH-Z20098) （LBH-Z20098）
黑龙江中医药大学研究生创新科研项目(2023yjscx010) （2023yjscx010）
深圳市龙岗区科技发展专项-医疗卫生科技计划项目(LGKCYLWS2020079) （LGKCYLWS2020079）
