中国中医药信息杂志2025，Vol.32Issue(4)：72-78,7.DOI:10.19879/j.cnki.1005-5304.202407063
青钱柳对棕榈酸钠诱导的HepG2细胞脂质沉积的影响及作用机制
Effects and Mechanism of Cyclocaryae Paliuri Folium in Sodium Palmitate-Induced Lipid Deposition in HepG2 Cells
摘要
Abstract
Objective To investigate the ameliorative effect and potential mechanism of Cyclocaryae Paliuri Folium in sodium palmitate-induced lipid deposition in HepG2 cells.Methods The effect of sodium palmitate and lyophilized powder of Cyclocaryae Paliuri Folium on the viability of HepG2 cells was determined by the CCK-8 method to determine the subsequent dosage administered.The HepG2 cells were divided into blank group,model group,pioglitazone group and Cyclocaryae Paliuri Folium low-,medium-and high-dosage group,the lipid deposition model of HepG2 cells was established using 350 μmol/L sodium palmitate,the medication group were given pioglitazone and low-,medium-and high-dosage of Cyclocaryae Paliuri Folium(100,250,500 μg/mL)for 12 h respectively.The intracellular lipid deposition was observed by Nile red staining and BODIPY493/503 fluorescent probe staining,the content of TNF-α and IL-6 in supernatant of cell culture medium were detected by ELISA,Western blot was used to detect PI3K,Akt,sterol-regulatory element binding protein-1(SREBP-1),fatty acid synthase(FAS),Bcl-2,Bax protein expression,qPCR was used to detect the mRNA expression of adipose triglyceride lipase(ATGL)and CD36.Results Compared with the blank group,the lipid deposition of HepG2 cells in the model group increased,TNF-α and IL-6 contents in the supernatant of cell culture medium significantly increased(P<0.01),the protein expressions of p-PI3K,p-Akt,SREBP-1,FAS and Bax in cells significantly increased,while the protein expression of Bcl-2 significantly decreased(P<0.01),the mRNA expression of ATGL significantly decreased,and the mRNA expression of CD36 significantly increased(P<0.01).Compared with the model group,the intracellular lipid deposition of the pioglitazone group and Cyclocaryae Paliuri Folium groups improved to varying degrees,the contents of TNF-α and IL-6 in the cell supernatant decreased,the expressions of p-PI3K,p-Akt,SREBP-1,FAS and Bax proteins decreased,the expression of Bcl-2 protein increased,the expression of ATGL mRNA increased,and the expression of CD36 mRNA decreased,with statistical significance in pioglitazone group and Cyclocaryae Paliuri Folium high-dosage group(P<0.05,P<0.01).Conclusion Cyclocaryae Paliuri Folium may ameliorate sodium palmitate-induced lipid deposition in HepG2 model cells by modulating the PI3K/Akt/SREBP-1/FAS signaling pathway and affecting triacylglycerol metabolism.关键词
青钱柳/HepG2细胞/脂质沉积/PI3K/AKt/SREBP-1/FAS信号通路Key words
Cyclocaryae Paliuri Folium/HepG2 cells/lipid deposition/PI3K/Akt/SREBP-1/FAS signaling pathway分类
医药卫生引用本文复制引用
刘艺璇,吴昊洋,阳晶晶,蔡昱哲,罗政,李定祥,邓奕辉..青钱柳对棕榈酸钠诱导的HepG2细胞脂质沉积的影响及作用机制[J].中国中医药信息杂志,2025,32(4):72-78,7.基金项目
国家重点研发计划(2022YFC3501202) （2022YFC3501202）
湖南省科技创新计划(2020RC4050) （2020RC4050）
湖南省卫生健康委科研课题(D202303067824) （D202303067824）
长沙市自然基金(kq2208188) （kq2208188）
湖南省中医药科研课题(B2023057) （B2023057）
