中国医院用药评价与分析2025，Vol.25Issue(4)：407-411,5.DOI:10.14009/j.issn.1672-2124.2025.04.006
阿纳其根醇提物通过 CREB/BDNF 信号轴改善围绝经期抑郁的机制研究
Mechanism of Ethanol Extract of Anacyclus Pyrethrum Root in Improving Perimenopausal Depression Through CREB/BDNF Signaling Axis
摘要
Abstract
OBJECTIVE:To investigate the neuroprotective and antidepressant effects of anacyclus pyrethrum root ethanol extract on perimenopausal depression model rats through cyclic adenosine phosphate response element binding protein(CREB)/brain-derived nerve growth factor(BDNF)signaling axis.METHODS:The rat model of perimenopausal depression was prepared by bilateral ovariectomy combined with chronic unpredictable mild stress.The experimental animals were randomly divided into the sham operation group,model group,positive drug group(fluoxetine 2 mg/kg+estrogen 0.15 mg/kg)and ethanol extract of anacyclus pyrethrum root group(low,medium and high dose groups were respectively given 10 mg/kg,20 mg/kg and 40 mg/kg),with 8 cases in each group and continuous treatment for 21 d.The behavioral changes of rats in each group were observed,and the behavioral changes of rats after administration were evaluated by open field test and forced swimming test.The changes of 5-hydroxytryptamine(5-HT)and cyclic adenosine monophosphate(cAMP)in serum of rats were detected by enzyme-linked immunosorbent assay.The mRNA expression levels of BDNF and CREB in hippocampus were determined by quantitative PCR(qPCR).Immunohistochemical analysis and Western blotting were used to detect CREB and BDNF in hippocampus of rats.RESULTS:Compared with the sham operation group,rats in the model group showed depressive-like behaviors,mainly including decreased autonomous and exploratory activities,prolonged behavioral despair time,and decreased serum 5-HT and cAMP levels;compared with the model group,the depression-like behavior of theethanol extract of anacyclus pyrethrum root group was improved,the ability of autonomous and exploratory activities was improved,the behavioral despair time was shortened,the serum 5-HT and cAMP levels increased,and the mRNA and protein expression levels of CREB and BDNF in hippocampus increased;among all dose groups,the improvement of the above indicators was more obvious in the high-doseethanol extract of anacyclus pyrethrum root group,the differences were statistically significant(P<0.05).CONCLUSIONS:The ethanol extract of anacyclus pyrethrum root can improve the depression-like behavior of perimenopausal depression model rats,and its mechanism is mainly related to the increase of 5-HT and cAMP levels.The improvement is also related to the up-regulation of CREB and BDNF protein expression.This study is of great value to elucidate the molecular mechanism of ethanol extract of anacyclus pyrethrum root in the treatment of perimenopausal depression,reveal the mechanism of the occurrence and development of perimenopausal depression,and realize the clinical treatment of depression.关键词
围绝经期抑郁/阿纳其根醇提物/环磷酸腺苷应答元件结合蛋白质/脑源性神经生长因子信号轴/5-羟色胺/环磷酸腺苷Key words
Perimenopausal depression/Ethanol extract of anacyclus pyrethrum root/Cyclic adenosine phosphate response element binding protein/brain-derived nerve growth factor signaling axis/5-hydroxytryptamine/Cyclic adenosine monophosphate分类
药学引用本文复制引用
买热木古·阿布都热依木,塔依尔·吐尔松,杨烨..阿纳其根醇提物通过 CREB/BDNF 信号轴改善围绝经期抑郁的机制研究[J].中国医院用药评价与分析,2025,25(4):407-411,5.基金项目
新疆维吾尔自治区自然科学基金资助项目(No.2023D01C419) （No.2023D01C419）
