种子2025，Vol.44Issue(3)：222-226,5.DOI:10.16590/j.cnki.1001-4705.2025.03.222
四引物扩增受阻突变体系PCR检测水稻白叶枯病抗性基因xa5的优化与应用
Optimization and Utilization of the Detection for Bacterial Blight Resistance Gene xa5 Molecular Marker in Rice with Tetra-primer ARMS PCR
摘要
Abstract
xa5 is a recessive gene for resistance to rice(Oryzasativa L.)bacterial blight.The main functional difference of Xa5 is that the TC bases at positions 116 and 117 of the molecular breeding for rice resistance to bacterial blight.In order to develop an easily identifiable and stable functional marker,based on the principle of Tetra Primer Amplification Refractory Mutation System PCR(TPARMS PCR),six sets of tetra-primer combinations were designed.By optimizing primer design and reaction system,a co-dominant marker(combined T5)was selected that could clearly distinguish xa5 homozygote(400/157 bp),Xa5 homozygote(400/289 bp)and heterozygote(400/289/157 bp)in 1％agarose gel electrophoresis.The marker was verified by F2 population to conform to Mendelian isolation rule(x2=0.93),and the disease resistance phenotype was consistent with the genotype.关键词
白叶枯病/xa5/四引物扩增受阻突变体系PCR/功能标记Key words
bacterial blight/xa5/tetra-primer ARMS-PCR/functional marker分类
农业科技引用本文复制引用
何开碧,武帅民,尤一航,李瑶,王平,印汉,夏志辉..四引物扩增受阻突变体系PCR检测水稻白叶枯病抗性基因xa5的优化与应用[J].种子,2025,44(3):222-226,5.基金项目
三亚崖州湾科技城科技专项(SCKJ-JYRC-2023-16) （SCKJ-JYRC-2023-16）
海南崖州湾种子实验室(B23YQ1512) （B23YQ1512）
中国种子集团有限公司"揭榜挂帅"项目(B23CQ15CP) （B23CQ15CP）
