中成药2025，Vol.47Issue(4)：1069-1076,8.DOI:10.3969/j.issn.1001-1528.2025.04.001
滇重楼皂苷近红外光谱快速定量分析模型建立
Model establishment for quantitative analysis of saponins of Paris polyphylla by near-infrared spectroscopy
摘要
Abstract
AIM To establish a rapid quantitative analysis model for saponins in Paris polyphylla var.yunnanensis(PPY)by near infrared spectroscopy.METHODS The contents of polyphyllins Ⅰ,Ⅱ,Ⅶ and there total content in PPY were determined by HPLC,while spectral data within the range of 10 000 to 4 000 cm-1 were collected.A quantitative analysis model was established by combining these data with partial least squares regression(PLSR).Multivariate scatter correction(MSC)and vector normalization(SNV)were applied prior to further preprocessing the spectra with original,first-order derivative(1stD),or second-order derivative(2ndD)treatments.Lastly,the model was optimized through non-smoothing(NS),Norris Derivative filtering(Nd),and Savitzky-Golay filtering(S-G)method.Model stability was evaluated based on correlation coefficients and variance.The predicted contents of each saponin component in the validation set samples were calculated.RESULTS The contents of polyphyllins Ⅰ,Ⅱ,Ⅶ were 0.42-17.98,0.46-10.44,0.23-3.86 mg/g,respectively.The total content ranged from 2.91 to 22.1 mg/g.The optimal parameters of three saponins were achieved when selecting the MSC+2ndD+S-G pretreatment method.The corresponding ratio of line segment length to segment gap was 13∶5,15∶5,11∶5,with correlation coefficients of 0.982,0.930,0.958,respectively.The root mean square errors of calibration(RMSEC)were 0.702,0.797,0.238,and the root mean square errors of prediction(RMSEP)were 1.120,0.835,0.304,respectively.The optimal parameters for the total content were obtained when selecting the MSC+2ndD+NS pretreatment method,with a correlation coefficient of 0.970,a RMSEC of 1.090,and a RMSEP of 1.740.CONCLUSION This accurate and rapid method can be used for detection of saponin contents in P.Polyphylla.关键词
滇重楼/皂苷/HPLC/近红外光谱/定量分析模型Key words
Paris polyphylla var.yunnanensis/saponins/HPLC/near infrared spectroscopy/quantitative analysis model分类
医药卫生引用本文复制引用
徐萍,米琪,罗文秀,陆尤,于梦雯,张萱,郑国伟,邱昌桂,陈佳..滇重楼皂苷近红外光谱快速定量分析模型建立[J].中成药,2025,47(4):1069-1076,8.基金项目
国家自然科学基金(82160718) （82160718）
云南省万人计划青年拔尖人才项目(YNWR-QNBJ-2020-257) （YNWR-QNBJ-2020-257）
云南省科技厅中医联合重点项目(202101AZ070001-163) （202101AZ070001-163）
云南省科技厅中医联合面上项目(202001AZ070001-047) （202001AZ070001-047）
