中成药2025，Vol.47Issue(4)：1077-1084,8.DOI:10.3969/j.issn.1001-1528.2025.04.002
基于UPLC-Q-TOF-MS/MS法、HPLC指纹图谱、含量测定评价覆盆子质量
Quality evaluation of Rubi Fructus based on UPLC-Q-TOF-MS/MS,HPLC fingerprints and content determination
摘要
Abstract
AIM To evaluate the quality of Rubi Fructus.METHODS UPLC-Q-TOF-MS/MS was adopted in the component identification,after which the HPLC fingerprints were established,cluster analysis,principal component analysis and orthogonal partial least squares discriminant analysis were used for chemical pattern recognition.and the contents of chlorogenic acid,ferulic acid,ellagic acid,isoquercitrin,kaempferol-3-O-rutinoside,astragalin,tiliroside quercetin,kaempferol were determined.RESULTS Total 34 constituents were identified.There were 19 common peaks in the fingerprints for 31 batches of medicinal materials with the similarities of more than 0.8.Wild varieties and cultivated varieties,and medicinal materials from different producing areas could be distinguished;4 principal components demonstrated the accumulative variance contribution rate of 84.142％;8 differential components were screened,2 of which were ellagic acid and astragalin.Ellagic acid and astragalin displayed higher contents in the wild varieties than those in the cultivated varieties(P＜0.05,P＜0.01).CONCLUSION UPLC-Q-TOF-MS/MS,HPLC fingerprints combined with content determination can be used for the quality control of Rubi Fructus.关键词
覆盆子/质量评价/UPLC-Q-TOF-MS/MS/HPLC指纹图谱/含量测定/化学模式识别Key words
Rubi Fructus/quality evaluation/UPLC-Q-TOF-MS/MS/HPLC fingerprints/content determination/chemical pattern recognition分类
医药卫生引用本文复制引用
李书晚,廖扬振,王龙飞,赵雅琴,范甲赫,魏渊,欧阳臻..基于UPLC-Q-TOF-MS/MS法、HPLC指纹图谱、含量测定评价覆盆子质量[J].中成药,2025,47(4):1077-1084,8.基金项目
中央本级重大增减支项目(2060302) （2060302）
