中成药2025，Vol.47Issue(4)：1084-1091,8.DOI:10.3969/j.issn.1001-1528.2025.04.003
不同产地西红花质量评价
Quality evaluation of Croci Stigma from different producing areas
摘要
Abstract
AIM To evaluate the quality of Croci Stigma from different producing areas.METHODS The analysis was performed on a 25 ℃ thermostatic Waters Acquity UPLC HSS T3 column(2.1 mm× 100 mm,1.8μm),with the mobile phase comprising of 0.1％phosphoric acid-acetonitrile flowing at 0.35 mL/min in a gradient elution manner,and the detection wavelengths were set at 254,440 nm.The UPLC fingerprints were established,after which orthogonal partial least squares discriminant analysis was performed,picrocrocin,crocin-Ⅰ,crocin-Ⅱ,crocin-Ⅲ,crocin-Ⅳ contents and chromaticity values(L*,a*,b*,E*ab)were determined,Pearson correlation analysis was adopted in the investigation of correlations between chromaticity values and internal constituent contents.RESULTS There were 14 common peaks in the fingerprints for 22 batches of medicinal materials with the similarities of more than 0.98.Various batches of medicinal materials were clustered into 2 types,7 quality difference components were screened.crocin-Ⅰ content in medicinal materials from different producing areas demonstrated significant differences(P＜0.05);the redder the color of medicinal material,the higher the contents of crocins.Picrocrocin,crocin-Ⅰ,crocin-Ⅱ,crocin-Ⅳ contents displayed highly significant correlations with colorimetric values(P＜0.01),while crocin-Ⅲ content exhibited no significant correlation with the latter(P＞0.05).CONCLUSION This accurate and reliable method can provide references for the quality control and color-quality relationship elucidation of Croci Stigma.关键词
西红花/产地/质量评价/UPLC指纹图谱/正交偏最小二乘法判别分析/含量测定/颜色/Pearson相关性分析Key words
Croci Stigma/producing areas/quality evaluation/UPLC fingerprints/orthogonal partial least squares discriminant analysis/content determination/color/Pearson correlation analysis分类
医药卫生引用本文复制引用
王瑞琪,沈一琦,许陈思菡,张永,张彤,丁越..不同产地西红花质量评价[J].中成药,2025,47(4):1084-1091,8.基金项目
上海市曙光学者项目(20SG43) （20SG43）
上海市优秀青年学术带头人项目(22XD1423000) （22XD1423000）
上海市中医药三年行动计划项目[ZY(2021-2023)-0215] （2021-2023）
上海中医药大学科技发展项目(23KFL039) （23KFL039）
上海中医药大学第十六批大学生创新创业项目(202310268147) （202310268147）
