中国中医药信息杂志2025，Vol.32Issue(5)：74-82,9.DOI:10.19879/j.cnki.1005-5304.202410324
基于全转录组学探讨冷冻消融联合养肺方对Lewis肺癌荷瘤小鼠的疗效及作用机制
Exploration on the Efficacy and Mechanism of the Combination of Cryoablation and Yangfei Prescription for the Treatment of Lewis Lung Cancer Bearing Mice Based on Whole Transcriptome
摘要
Abstract
Objective To investigate the efficacy and mechanism of the combination of cryoablation and Yangfei establish the Lewis lung cancer mouse model,and randomly divided into model group,cryoablation group,cryoablation+cisplatin group,cryoablation+Yangfei Prescription low-,medium-and high-dosage groups,with 5 mice in each group.Sham-operation was performed in the model group,cryoablation+cisplatin group were given intraperitoneal injection of cisplatin 3 mg/kg after cryoablation,cryoablation+Yangfei Prescription low-,medium-and high-dosage groups were given 1.64,3.28,6.56 g/kg Yangfei Prescription by gavage after cryoablation respectively,and the model group was given equal volume of normal saline by gavage for 14 consecutive days.Tumor volume and tumor mass were measured,the morphology of tumor tissue was observed by HE staining,the expression of Ki-67 protein in tumor tissue was detected by immunohistochemistry.The differentially expressed genes in tumor tissues after cryoablation combined with Yangfei Prescription intervention were analyzed by whole transcriptome sequencing,GO and KEGG pathway enrichment analysis was performed on differentially expressed genes,and the lncRNA/circNA-mediated ceRNA network was constructed.Differentially expressed genes were verified using RT-qPCR.Results The main active components of Yangfei Prescription were terpenoids,flavonoids and aldehydes.Compared with the model group,tumor volume and tumor mass decreased(P<0.05)in cryoablation+Yangfei Prescription low-,medium-and high-dosage groups and cryoablation+cisplatin group;cryoablation combined with Yangfei Prescription could destroy the structure of tumor tissue and inhibit cell proliferation(P<0.05).A total of 1 585 differentially expressed genes(1 160 mRNA,225 lncRNA,155 miRNA and 45 circRNA)of the model group and cryoablation+Yangfei Prescription high-doseage group were obtained.GO enrichment analysis mainly involved biological processes such as immune system response and cell proliferation,cellular components such as protein complex and cell junction,molecular functions such as transcription regulator activity and molecular function regulator;KEGG pathway enrichment mainly occurred in cancer-related pathways such as PI3K-Akt signaling pathway,Th17 cell differentiation,mTOR signaling pathway,FOXO signaling pathway,etc.The lncRNA-mediated ceRNA network was composed of 97 lncRNA,73 miRNA and 417 mRNA,and the circRNA-mediated ceRNA network was composed of 26 circRNA,68 miRNA and 157 mRNA.RT-qPCR validated the expressions of 15 differentially expressed genes,which was consistent with the sequencing results.Conclusion Cryoablation combined with Yangfei Prescription can inhibit the tumor growth of Lewis lung cancer bearing mice,destroy the structure of tumor tissue,inhibit cell proliferation,the mechanism may be related to the regulation of Gm38393/miRNA-136-5p/Zfp704 axis.关键词
养肺方/非小细胞肺癌/全转录组测序/竞争性内源RNA/小鼠Key words
Yangfei Prescription/non-small cell lung cancer/whole transcriptome sequencing/ceRNA/mice分类
医药卫生引用本文复制引用
王潇凡,张墨涵,刘殿娜,林事成,李越,李依聪,李泉旺..基于全转录组学探讨冷冻消融联合养肺方对Lewis肺癌荷瘤小鼠的疗效及作用机制[J].中国中医药信息杂志,2025,32(5):74-82,9.基金项目
国家自然科学基金青年科学基金(82104957) （82104957）
中央高校基本科研业务费专项资金资助北京中医药大学重点攻关项目(2020-JYB-ZDGG-127) （2020-JYB-ZDGG-127）
