中国中医药信息杂志2025，Vol.32Issue(5)：83-89,7.DOI:10.19879/j.cnki.1005-5304.202408465
苓桂气化方抗射血分数保留的心力衰竭心肌炎症和纤维化的机制研究
Study on the Mechanism of Linggui Qihua Prescription against Myocardial Inflammation and Fibrosis in Heart Failure with Preserved Ejection Fraction
摘要
Abstract
Objective To investigate the effects and mechanism of Linggui Qihua Prescription against myocardial inflammation and fibrosis in heart failure with preserved ejection fraction(HFpEF).Methods Totally 40 spontaneously hypertensive rats(SHR)were equally divided into model group,Entresto group(18 mg/kg)and Linggui Qihua Prescription low-and high-dosage groups(3.87,7.74 g/kg),the HFpEF model was induced using a 16-week high fat-salt-sugar diet and 8-week streptozotocin intraperitoneal injection.Ten WKY rats(WKY group)and 10 SHR(SHR group)served as control.After successful modeling,the groups were subjected to a 6-week corresponding intervention.Left ventricular end diastolic diameter(LVEDD),left ventricular end-diastolic volume(LVEDV),relative ventricular wall thickness(RWT),left ventricular fractional shortening(LVFS),isovolumic relaxation time(IVRT),mitral annular early and late diastolic velocities of motion(eʹ and aʹ),global longitudinal strain(GLS)and global longitudinal strain rate(GLSr)were measured by echocardiography;serum tumor necrosis factor-α(TNF-α)and soluble growth stimulation expressed gene 2 protein(sST2)contents were detected by ELISA;the morphology of myocardial tissue was observed by HE staining;myocardial fibrosis was assessed by Masson staining;the mRNA and protein expressions of myocardial tissue vascular cell adhesion molecule-1(VCAM-1),intercellular adhesion molecule-1(ICAM-1),growth differentiation factor-15(GDF-15)and sST2 were detected by qPCR and Western blot.Results Compared with the WKY group and SHR group,the model group exhibited significant increase in LVEDV,RWT,IVRT,along with significant decrease in eʹ,aʹ and absolute values of GLS and GLSr(P<0.05,P<0.01),the serum contents of TNF-α and sST2 significantly increased(P<0.01);there was pronounced myocardial inflammatory infiltration and collagen fiber deposition,while the mRNA and protein expression of VCAM-1,ICAM-1,GDF-15 and sST2 significantly increased(P<0.05,P<0.01).Compared with the model group,Entresto group and Linggui Qihua Prescription low-and high-dosage groups demonstrated significant decrease in LVEDV,RWT,IVRT,along with significant increases in eʹ,aʹ and absolute values of GLS and GLSr(P<0.05,P<0.01),the serum contents of TNF-α and sST2 significantly decreased(P<0.01);myocardial inflammatory infiltration and collagen fiber deposition were reduced,the mRNA and protein expressions of ICAM-1,GDF-15 and sST2 decreased in Linggui Qihua Prescription high-dosage group(P<0.01).Conclusion Linggui Qihua Prescription may inhibit chronic inflammation and fibrosis of the myocardium in HFpEF rats by regulating the expressions of ICAM-1,VCAM-1,GDF-15 and sST2,improving cardiac remodeling and functional impairment.关键词
射血分数保留的心力衰竭/苓桂气化方/黏附分子/生长分化因子-15/可溶性生长刺激表达基因2蛋白/大鼠Key words
heart failure with preserved ejection fraction/Linggui Qihua Prescription/adhesion molecules/growth differentiation factor-15/soluble growth-stimulated expressed gene 2 protein/rats分类
医药卫生引用本文复制引用
石玉姣,樊民,刘思雨,董国菊..苓桂气化方抗射血分数保留的心力衰竭心肌炎症和纤维化的机制研究[J].中国中医药信息杂志,2025,32(5):83-89,7.基金项目
国家自然科学基金(82074423) （82074423）
中国中医科学院西苑医院能力提升项目(XYZX0201-02) （XYZX0201-02）
中国中医科学院科技创新工程(CI2021A00903) （CI2021A00903）
