中国中医药信息杂志2025，Vol.32Issue(5)：141-147,7.DOI:10.19879/j.cnki.1005-5304.202411454
炙红芪-醋莪术配伍前后化学成分及指纹图谱研究
Study on Chemical Constituents and Fingerprints of Hedysari Radix Praeparata Cum Melle and Vinegar Processed Curcumae Rhizoma before and after Compatibility
摘要
Abstract
Objective Through studying the chemical composition changes before and after the compatibility of Hedysari Radix Paeparata Cum Melle(HRPCM)-vinegar processed Curcumae Rhizoma(VPCR);To discuss the significance of the compatibility of HRPCM and VPCR;To establish the fingerprints before and after their compatibility.Methods ZORBAX Eclipse Plus C18 column was used;acetonitrile-0.01%phosphoric acid water was set as mobile phase,with gradient elution;column temperature was 30℃;detection wavelength was 214 nm;sample injection was 10 μL,which was used to investigate the content difference of major chemical components such as vanillic acid,calycosin-O-β-D-glucopyranoside,ononin,calycosin,onocerin,curdione,cincumol and germacrone,and establish the fingerprint of HRPCM,VPCR and HRPCM-VPCR.Results HPLC chromatographic conditions were established for the determination of 8 components in HRPCM-VPCR.Meanwhile,fingerprints were established before and after the compatibility of HRPCM-VPCR.26 common peaks were identified,among which 11 components such as vanillic acid were derived from HRPCM,14 components such as curcuma zedoariae were derived from VPCR,and 1 component was shared by both.Conclusion The material basis of the compatibility of HRPCM-VPCR differs from that of HRPCM and VPCR.The content of most chemical components decreases while the content of some components increases.The established HPLC method for content determination and fingerprint is simple,stable and reproducible,which can be used to evaluate and control the quality of HRPCM and VPCR.关键词
炙红芪/醋莪术/高效液相色谱法/配伍/化学成分/指纹图谱Key words
Hedysari Radix Paeparata Cum Melle/vinegar processed Curcumae Rhizoma/HPLC/compatibility/chemical composition/fingerprint分类
医药卫生引用本文复制引用
李越峰,窦芬瑜,张转红,吕瑞龙,柴梦娜,马定财,王毛毛,王哲,严兴科..炙红芪-醋莪术配伍前后化学成分及指纹图谱研究[J].中国中医药信息杂志,2025,32(5):141-147,7.基金项目
国家自然科学基金(81960713、82160750) （81960713、82160750）
甘肃省教育厅产业支撑计划项目(2021CYZC-21) （2021CYZC-21）
甘肃省科技计划(创新基地与人才计划)基础研究创新群体项目(21JR7RA569) （创新基地与人才计划）
甘肃省省级人才项目-中药传统炮制技术传承创新团队建设与示范推广(2024年) （2024年）
甘肃省联合科研基金资助项目(24JRRA882) （24JRRA882）
高等学校产业支撑计划基金资助项目(2024CYZC-41) （2024CYZC-41）
