中成药2025，Vol.47Issue(4)：1122-1128,7.DOI:10.3969/j.issn.1001-1528.2025.04.009
苁蓉散对阿尔茨海默病大鼠神经元细胞凋亡和Bax/Bcl-2/Caspase3信号通路的影响
Effects of Congrong San on neuronal apoptosis and Bax/Bcl-2/Caspase3 signaling pathway in a rat model of Alzheimer's disease
摘要
Abstract
AIM To investigate the effects of Congrong San on neuronal apoptosis and the Bax/Bcl-2/Caspase3 signaling pathway in a rat model of Alzheimer's disease(AD).METHODS A total of 60 2-month-old SD male rats were randomly divided into the blank group,the model group,the memantine hydrochloride group(0.025 g/kg)and low-dose,medium-dose and high-dose Congrong San groups(4.62,9.24,18.48 g/kg).All groups except the control group received stereotactic intracerebral injection of Aβ1-42 to establish AD models.Following the successful modeling,each group received its corresponding intragastric administration once daily for 28 consecutive days.After the administration,the rats had their learning and memory ability detected by the morris water maze test;their hippocampal neuronal morphology observed with HE and Nissl staining;their hippocampal neuronal apoptosis observed with TUNEL staining;and their hippocampal expressions of amyloid precursor protein(APP),β-site APP-cleaving enzyme 1(BACE1),and apoptosis-related proteins Bax,Bcl-2 and Caspase3 detected with Western blot assay.RESULTS Compared with the model group,the groups intervened with medium-dose and high-dose Congrong San exhibited improved learning and memory performance,alleviated hippocampal neuronal damage,increased Nissl body count(P＜0.01),reduced hippocampal apoptosis rate(P＜0.05,P＜0.01),decreased protein expressions of APP,BACE1,Bax and cleaved-Caspase3/Caspase3 ratio(P＜0.05,P＜0.01),and elevated Bcl-2 expression(P＜0.01).CONCLUSION Congrong San mitigates cognitive impairment,hippocampal neuronal damage,and apoptosis in AD rats,probably through inhibition of the Bax/Bcl-2/Caspase3 signaling pathway activation.关键词
苁蓉散/阿尔茨海默病/海马/神经元损伤/凋亡/淀粉样前体蛋白(APP)/β位点淀粉样前体蛋白裂解酶1(BACE1)Key words
Congrong San/Alzheimer's disease/hippocampus/neuronal damage/apoptosis/amyloid precursor protein(APP)/β-site amyloid precursor protein cleaving enzyme 1(BACE1)分类
医药卫生引用本文复制引用
蔡元钦,向阳,龙清华,王曦,张婧凡,曾楚华..苁蓉散对阿尔茨海默病大鼠神经元细胞凋亡和Bax/Bcl-2/Caspase3信号通路的影响[J].中成药,2025,47(4):1122-1128,7.基金项目
国家自然科学基金(82060831) （82060831）
