中成药2025，Vol.47Issue(4)：1137-1143,7.DOI:10.3969/j.issn.1001-1528.2025.04.011
白背三七多糖对干酵母联合腺嘌呤所致痛风性肾病大鼠的影响
Effects of Gynura divaricate polysaccharide on gouty nephropathy induced by dry yeast combined with adenine in rats
摘要
Abstract
AIM To study the protective effect of Gynura divaricate polysaccharide on gouty nephropathy(GN)induced by dry yeast combined with adenine in rats.METHODS Sixty male SD rats were randomly divided into the normal group,the model group,the allopurinol group(42 mg/kg),and the low-dose,medium-dose and high-dose G.divaricate polysaccharide groups(140,280,560 mg/kg).All the rats except those of the normal group were induced into GN models by intragastrical dosing of yeast(5 g/kg)and adenine(100 mg/kg)and intervened with corresponding drug administration simultaneously.After 35 days,the rats had their levels of creatinine(Cr)and uric acid(UA)in serum and urine detected and their fraction excretion of uric acid(FEUA)value determined;their kidney mass and volume measured and their levels of kidney index and density calculated;their renal pathological changes checked by HE staining;their renal GLUT9,URAT1,ABCG2 and OAT1 mRNA expressions dectected by RT-qPCR;and their renal GLUT9,URAT1,ABCG2 and OAT1 protein expressions dectected by Western blot.RESULTS Compared with the model group,each dose of G.divaricate polysaccharide group displayed decreased levels of kidney mass,kidney volume and kidney index(P＜0.01);increased levels of kidney density(P＜0.05,P＜0.01);decreased serum levels of UA and Cr(P＜0.01);increased urine levels of UA and Cr(P＜0.01);increased FEUA value(P＜0.01);decreased GLUT9,URAT1 mRNA and protein expressions(P＜0.05,P＜0.01);and increased ABCG2,OAT1 mRNA and protein expressions(P＜0.05,P＜0.01);and more alleviated renal histological aberrations.CONCLUSION G.divaricate polysaccharide exerts good protective effects against yeast/adenine-induced GN in rats probably through down-regulating protein expression of GLUT9,URAT1 and up-regulating ABCG2 and OAT1.关键词
白背三七多糖/痛风性肾病/别嘌醇/腺嘌呤/干酵母Key words
Gynura divaricate polysaccharide/gouty nephropathy/allopurino/adenine/dry yeast分类
医药卫生引用本文复制引用
植春婷,魏玉华,张淼,刘祖锭,朱华,许立拔..白背三七多糖对干酵母联合腺嘌呤所致痛风性肾病大鼠的影响[J].中成药,2025,47(4):1137-1143,7.基金项目
广西壮族自治区科学技术厅广西中医药大学-中南大学壮瑶药研究联合实验室项目(桂科计字[2021]238号) （桂科计字[2021]238号）
广西产5种壮瑶药材质量标准与产品开发研究项目(桂科计字[2021]196号) （桂科计字[2021]196号）
广西壮族自治区科学技术厅广西壮瑶药重点实验室项目(桂科基字[2014]32号) （桂科基字[2014]32号）
广西壮族自治区发展改革委员会广西壮族自治区民族药资源与应用工程研究中心项目(桂发改高技函[2020]2605号) （桂发改高技函[2020]2605号）
广西壮族自治区教育厅壮瑶药协同创新中心项目(桂教科研[2013]20号) （桂教科研[2013]20号）
广西第八批特聘专家项目(桂人才通字[2019]13号) （桂人才通字[2019]13号）
