中成药2025，Vol.47Issue(4)：1143-1149,7.DOI:10.3969/j.issn.1001-1528.2025.04.012
广东石豆兰水提物对D-半乳糖致大鼠肝组织氧化损伤及Nrf2/HO-1/NQO-1信号通路的影响
Effects of aqueous extract of Bulbophyllum kwangtungense on liver damage and Nrf2/HO-1/NQO-1 signaling pathway in a rat model of D-galactose-induced oxidative stress
摘要
Abstract
AIM To investigate the protective effects of the aqueous extract of Bulbophyllum kwangtungense Schltr.on D-galactose(D-gal),an oxidative stress-induced liver damage in rats.METHODS 60 Male SD rats were randomly divided into the normal control group,the model group,the resveratrol group(20 mg/kg),and low-dose,medium-dose,and high-dose B.kwangtungense aqueous extract groups(750,1 500 and 3 000 mg/kg).Except for those of the normal control group,the rats of the other groups were subcutaneously injected with D-gal(200 mg/kg)in the back of neck to establish a liver damage model.Simultaneously,each group underwent the corresponding drug administration by gavage(10 mL/kg)once daily for 42 days.After the treatment period,the rats had their peripheral blood and liver tissues collected for the calculation of the liver index;their serum AST and ALT activities,hepatic SOD and GSH-Px activities,and MDA levels measured;their hepatic pathological changes observed using HE staining;their hepatic mRNA expressions of Nrf2,NQO-1 and HO-1 detected by RT-qPCR;and their hepatic protein expressions of Nrf2,NQO-1,HO-1,Bax,Bcl-2 and cleaved Caspase-3 measured by Western blot.RESULTS Compared with the model group,the groups intervened with medium-dose or high-dose B.kwangtungense aqueous extract showed increased liver index level(P＜0.01);decreased serum AST and ALT activities(P＜0.01);increased hepatic SOD and GSH-Px activities(P＜0.05,P＜0.01);reduced MDA levels(P＜0.01);alleviated pathological liver damage and increased hepatic mRNA and protein expressions of Nrf2,NQO-1 and HO-1(P＜0.05,P＜0.01);and reduced protein expressions of cleaved Caspase-3 and the Bax/Bcl-2 ratio as well(P＜0.05,P＜0.01).CONCLUSION The aqueous extract of B.kwangtungense is protective of rats with D-gal-induced liver injury,and the underlying mechanism may associate with the inhibition of oxidative stress and the suppression of hepatocellular apoptosis mediated by the activation of the Nrf2/HO-1/NQO-1 signaling pathway.关键词
广东石豆兰/水提物/D-gal/氧化应激/凋亡/Nrf2/HO-1/NQO-1信号通路Key words
Bulbophyllum kwangtungense Schltr./aqueous extract/D-gal/oxidative stress/apoptosis/Nrf2/HO-1/NQO-1 signaling pathway分类
医药卫生引用本文复制引用
王冰冰,朱华,张淼,许立拔,韦春合,黄婷..广东石豆兰水提物对D-半乳糖致大鼠肝组织氧化损伤及Nrf2/HO-1/NQO-1信号通路的影响[J].中成药,2025,47(4):1143-1149,7.基金项目
广西科技基地和人才专项(桂科AD21238031) （桂科AD21238031）
广西重点研发计划项目(桂科AB21196016) （桂科AB21196016）
广西中医药重点学科壮药学(GZXK-Z-20-64) （GZXK-Z-20-64）
广西一流学科中药学(民族药学)(桂教科研[2018]12号) （民族药学）
