浙江农业学报2025，Vol.37Issue(3)：689-700,12.DOI:10.3969/j.issn.1004-1524.20240352
基于响应面分析的养殖水产品中高风险喹诺酮类抗生素残留及其基质效应研究
Study for the determination of high-risk quinolone antibiotics residues and its matrix effect in cultured aquatic products by UPLC-MS/MS based on response surface analysis
摘要
Abstract
In order to establish an general,efficient and accurate residue analysis method for high risk quinolone an-tibiotics in cultured aquatic products,such as Larimichthys crocea,Carassius auratus,Pelodiscus sinensis,etc,all the samples were extracted using ultrasonic and vibrating extraction coupling,and then cleaned by liquid-liquid ex-traction and determined by UPLC-MS/MS.The optimal pretreatment conditions were determined by combining hydro-chloric acid concentration in extraction reagent,oscillation time,ultrasonic time,nitrogen blowing pressure,temper-ature with Box-Behnken response surface analysis.The matrix effects of the target objects were evaluated in this stud-y,and the compensation method of matrix effects in Larimichthys crocea,Carassius auratus,Pelodiscus sinensis,Penaeus vannemei,Scylla serrata and its gonad,and Sinonovacula constricta was discussed by isotope internal stand-ard and matrix matching curve.The results showed that the average recovery rate of 6 target substances was 82.20％-118.00％.The linear correlation coefficients were above 0.997 in spiked range of 0.2-20 ng·mL-1.The limits of detection and limits of quantitation were 0.5 μg·kg-1 and 1.0 μg·kg-1,respectively.It showed that the method met the requirements for the analysis of antibiotic residues in cultured aquatic products.To varying degrees,the ma-trix of Penaeus vannamei,Scylla serrata gonad and Larimichthys crocea inhibited the 6 target substances.The matrix matching curve and internal standard method could compensate the matrix effect effectively.关键词
响应面分析/超高效液相色谱串联质谱/养殖水产品/高风险/喹诺酮/基质效应评价Key words
response surface/UPLC-MS/MS/cultured aquatic product/high risk/quinolone/matrix effects evalu-ation分类
农业科技引用本文复制引用
徐汇镔,朱洁,周朝生,胡园,陆荣茂..基于响应面分析的养殖水产品中高风险喹诺酮类抗生素残留及其基质效应研究[J].浙江农业学报,2025,37(3):689-700,12.基金项目
浙江省基础公益研究计划(LTGC23B050007) （LTGC23B050007）
