中药药理与临床2025，Vol.41Issue(4)：78-83,6.
基于长时程增强机制研究生姜提取物对染铝大鼠学习记忆功能和神经细胞结构的干预作用
Intervention of Ginger Extract on Learning and Memory Function and Nerve Cell Structure in Aluminum-Exposed Rats Based on Long-Term Potentiation Mechanism
摘要
Abstract
Objective:In this study,different doses of ginger extract were used to intervene in aluminum-exposed rats.The learning and memory function and pathological changes of the hippocampus in aluminum-exposed rats were observed,and the expression levels of long-term potenti-ation-related molecules were detected to explore its mechanism.Methods:Sixty SD male rats were randomly divided into normal control group,model control group,donepezil hydrochloride group,and ginger extract groups of 0.1 g/kg,0.2 g/kg,and 0.4 g/kg,with 10 rats in each group.The normal control group was free to drink drinking water without AlCl3,and the other groups were free to drink drinking water containing 10 mg/mL AlCl3.After three months,the normal control group and the model control group were intragastrically administered with normal saline,and the other groups were intragastrically administered with corresponding drugs for four weeks.The pathological morphology of the rat hippocampus was observed by transmission electron microscope.The protein and mRNA expression levels of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor 1(GluR1),N-methyl-D-aspartate receptor 1(NMDAR1),calmodμLin-dependent protein kinase II(CaMKII),neuronal nitric oxide synthase(nNOS)and protein kinase C(PKC)in hippocampus of rats were detected by real-time fluores-cence quantitative polymerase chain reaction(Real-time PCR)and Western blot,respectively.Results:Compared with the normal control group,the escape latency of the model control group was significantly increased on the 4th and 5th day,while the number of crossing platforms and the residence time in the target quadrant were significantly reduced(P<0.01).The hippocampal neurons and synaptic structures showed abnormal changes,while the expression levels of GluR1,NMDAR1,CaMKII,nNOS,and PKC were decreased(P<0.05).Compared with the model control group,on the 4th and 5th day,the escape latency was significantly decreased in the donepezil hydrochloride group and ginger ex-tract groups of 0.2 g/kg and 0.4g/kg,while the number of crossing platforms and the residence time in the target quadrant were significantly increased(P<0.05).The escape latency was reduced on the 5th day in the ginger extract group of 0.1 g/kg.The pathological morphology of brain tissue in each administration group was improved to varying degrees.The improvement of the ginger extract groups of 0.2 and 0.4 g/kg was better than that of the ginger extract group of 0.1 g/kg and the donepezil hydrochloride group.The expression of GluR1 and PKC in the donepezil hydrochloride group was increased(P<0.05),and the expression of nNOS in the ginger extract group of 0.1 g/kg was increased(P<0.05);the expressions of GluR1,NMDAR1,CaMKII,nNOS,and PKC in the ginger extract group of 0.2 g/kg were increased(P<0.05).The expressions of GluR1,nNOS,and PKC in the ginger extract group of 0.4 g/kg were increased(P<0.05).Compared with that in the donepezil hydrochloride group,the expression of nNOS in the ginger extract group of 0.1 g/kg was higher(P<0.05),and the ex-pressions of NMDAR1 and nNOS in the ginger extract group of 0.2 g/kg were higher(P<0.05).The expressions of GluR1,nNOS,and PKC in the ginger extract group of 0.4 g/kg were higher(P<0.05).Conclusion:Ginger extract and clinical donepezil hydrochloride intervention can improve the learning and memory ability of aluminum-exposed rats.Ginger extract can inhibit nerve cells and synaptic damage.The mechanism of action may be related to the downregulating of the expressions of the antagonism of aluminum-induced LTP-related factors GluR1,NMDAR1,CaMKII,nNOS,and PKC.关键词
生姜提取物/铝/神经毒性/长时程增强/钙调蛋白依赖性蛋白激酶II/N-甲基-D-天冬氨酸受体1/α-氨基-3-羟基-5-甲基-4-异恶唑丙酸受体1/蛋白激酶/神经元型一氧化氮合酶Key words
Ginger extract/Aluminum/Neurotoxicity/Long-term potentiation/CaMKII/NMDAR1/GluR1/PKC/nNOS引用本文复制引用
谢晶,蒋小云,任丹,覃黎黎,凌健安,麦志丹,韦益宇,杨莉..基于长时程增强机制研究生姜提取物对染铝大鼠学习记忆功能和神经细胞结构的干预作用[J].中药药理与临床,2025,41(4):78-83,6.基金项目
广西壮族自治区卫生健康委员会自筹经费科研课题(合同号:Z20180271). （合同号:Z20180271）
