转化医学杂志2025，Vol.14Issue(3)：123-128,6.DOI:10.3639/j.issn.2095-3097.2025.03.023
甲状腺乳头状癌中长链非编码RNA NEAT1和微小RNA-34a表达及临床意义
Expression and Clinical Significance of Long Non-Coding RNA NEAT1 and microRNA-34a in Papillary Thyroid Cancer
摘要
Abstract
Objective To investigate the expression levels and clinical significance of long non-coding RNA(Ln-cRNA)nuclear-enriched abundant transcript 1(NEAT1)and microRNA(miR)-34a in papillary thyroid cancer(PTC)tissues.Methods A total of 89 patients with PTC who were admitted to our hospital from November 2016 to June 2018 were select-ed.Both PTC tissues and paracancerous tissues(PT)were collected during surgery.Real-time fluorescence quantitative RT-PCR was used to detect the expression levels of LncRNA NEAT1 and miR-34a in PTC tissues.Pearson's correlation anal-ysis was performed to analyze the correlation between LncRNA NEAT1 and miR-34a expression levels.The Kaplan-Meier survival curve was used to analyze the relationship between the relative expression of LncRNA NEAT1,miR-34a and PTC prognosis.Cox regression models were used to analyze the factors affecting the prognosis of PTC patients.Results The rel-ative expression level of LncRNA NEAT1 in PTC tissues(4.19±0.90)was significantly higher than that in PT(1.36±0.34),while the miR-34a expression(0.43±0.12)was lower than that in PT(1.33±0.36)(P<0.05).There were significant differ-ences in the expression quantity of LncRNA NEAT1 and miR-34a in the differentiation degree of cancer tissues,and there were also significant differences in the expression quantity of miR-34a in lymph node metastasis(P<0.05).The expression levels of LncRNA NEAT1 and miR-34a in PTC tissues are negatively correlated(r=-0.516,P<0.05).The high expression of LncRNA NEAT1 of patients 3-year survival rate was lower than that of patients with low expression(P<0.05),and the high expression of miR-34a of patients 3-year survival rate was lower than that of patients with low expression(P<0.05).Low ex-pression of miR-34a,high expression of LncRNA NEAT1,and low differentiation are independent risk factors affecting the prognosis of PTC patients(P<0.05).Conclusion LncRNA NEAT1 is highly expressed in PTC tissues,while miR-34a is low expressed,showing a negative correlation.Both molecules may serve as novel biomarkers for prognosis evaluation of PTC.关键词
甲状腺乳头状癌/长链非编码RNA核富含丰富的转录本1/微小RNA-34a/预后Key words
Papillary thyroid cancer/Long non-coding RNA nuclear-enriched abundant transcript 1/MicroRNA-34a/Prognosis分类
医药卫生引用本文复制引用
崔仪,汪阔,贾晓斌,雷珍珍,向辉..甲状腺乳头状癌中长链非编码RNA NEAT1和微小RNA-34a表达及临床意义[J].转化医学杂志,2025,14(3):123-128,6.基金项目
陕西省卫生健康科研基金项目(2018C006) （2018C006）
