中国农业科技导报2025，Vol.27Issue(5)：49-60,12.DOI:10.13304/j.nykjdb.2023.0847
低温胁迫对玉米幼苗光合性能、抗氧化酶系统及相关基因表达的影响
Effect of Low Temperature Stress on Photosynthetic Performance,Antioxidant Enzyme System and Related Gene Expression in Maize Seedlings
摘要
Abstract
To explore the response mechanism of maize seedlings to low temperature stress,the low-temperature sensitive GS2264 and the low-temperature tolerant LY2030 were used as materials,which were cultured at 10(LT)and 22℃(CK)for 7 d.And the changes of seedling growth and photosynthetic performances,reactive oxygen species,osmotic regulation,antioxidant enzymes activities,and the expression levels of antioxidant enzymes related genes in leaves were analyzed.The results showed that low temperature stress inhibited the growth and development of maize seedlings.Compared with CK treatment,the seedling length,root length,seedling fresh weight,root fresh weight,and root-shoot ratio of LT treatment significantly decreased by 7.06%,19.60%,18.75%,24.32%and 7.79%,respectively,and the net photosynthetic rate,stomatal conductance,transpiration rate,rubisco activity,water use efficiency,Fv/Fm and Fv/Fo significantly decreased by 42.88%,27.09%,15.23%,59.38%,29.78%,31.90%and 32.88%,respectively,however,intercellular CO2 concentration,O·-2 production rate and the contents of H2O2,proline,malondialdehyde significantly increased by 67.27%and 119.55%,154.04%,55.19%,the activities of SOD,POD,CAT,APX significantly increased by 46.66%,96.53%,158.44%,4.34%,while GR activity showed a slight decreased of 3.34%.The correlation and principal component analyses showed that a complex and network relationship among traits of maize seedlings.Further analysis showed the relative expression levels of 8 antioxidant oxidase related genes were significantly up-regulated under LT treatment,which indicated they could be used as candidate genes for improving cold resistance in maize.Above results laid a foundation for systematically revealing the cold-resistant response mechanism of maize seedlings and the improvement of cold-resistant maize germplasm.关键词
玉米幼苗/低温胁迫/光合性能/活性氧/抗氧化酶/基因表达水平Key words
maize seedling/low-temperature stress/physiological response/reactive oxygen species/antioxidant enzymes/gene expression level分类
农业科技引用本文复制引用
毛桃桃,赵小强,柏小栋,余斌..低温胁迫对玉米幼苗光合性能、抗氧化酶系统及相关基因表达的影响[J].中国农业科技导报,2025,27(5):49-60,12.基金项目
甘肃省科技重大专项(22ZD6NA009) （22ZD6NA009）
国家自然科学基金项目(32360478,32060486) （32360478,32060486）
省部共建干旱生境作物学国家重点实验室开放基金项目(GSCS-2023-Z1,GSCS-2023-08) （GSCS-2023-Z1,GSCS-2023-08）
甘肃省青年科技基金项目(21JR7RA840). （21JR7RA840）
