中国农业科技导报2025，Vol.27Issue(5)：61-71,11.DOI:10.13304/j.nykjdb.2024.0118
盐碱胁迫下燕麦蛋白组及代谢组联合分析
Proteinome and Metabolome Combined to Analyze the Response Mechanism of Oat to Saline-alkali Stress
摘要
Abstract
Salt-alkali stress is one of the main abiotic stresses limits plant growth.Planting oats in saline-alkali land allows the oat stems to absorb salt from the soil and store it in oat straw to repair the saline-alkali land.In order to clarify the effects of salt-alkali stress on oat proteome and metabolome,four-week-old oats were treated with 6,12,24 and 48 h salt-alkali stress,and the physiological and biochemical properties of oats after salt-alkali stress were measured,and its proteome and metabolome were analyzed.The results showed that,with the prolongation of salt-alkali stress time,malondialdehyde content of oat increased overall,the proline content and the activities of superoxide dismutase,peroxidase,catalase generally showed an upward trend.Proteomic and metabolomic sequencing identified a total of 7 603 differential proteins,including 3 217 up-regulated,4 386 down-regulated and 855 differential metabolites.They were mainly involved in pathways such as sugar metabolism,amino acid metabolism,photosynthesis and phenylpropanoid biosynthesis.Among them,lycopeneβ-cyclase,cyclooxygenase and zeaxanthin were key enzymes related to photosynthesis.In summary,salt-alkali stress enhanced the accumulation of metabolites such as sugars,nucleosides and amino acids.Ribosomes,phenylpropanoid biosynthesis,glyoxylic acid and dicarboxylic acid metabolism,and carbon fixation in photosynthetic organisms of leaves were the main pathways responsed salt-alkali stress.Above resltus provided effective informations for the restoration of saline-alkali soil and the reconstruction of ecosystems,and laid a theoretical foundation for the genetic improvement and cultivation of oat stress resistance.关键词
燕麦/蛋白质组/代谢组/联合分析/盐碱胁迫Key words
oat/proteomics/metabolomics/joint analysis/saline-alkali stress分类
农业科技引用本文复制引用
凌磊,蒋慧欣,李铭婧,殷亚杰,陈乃钰,赵晓菊..盐碱胁迫下燕麦蛋白组及代谢组联合分析[J].中国农业科技导报,2025,27(5):61-71,11.基金项目
黑龙江省自然科学项目基金(LH2021C001) （LH2021C001）
大学生创新创业训练项目(202310235A001) （202310235A001）
大庆市指导科技计划项目(zd-2023-12) （zd-2023-12）
黑龙江省省属高等学校基本科研业务费项目(2023-KYYWF-0026). （2023-KYYWF-0026）
