中国农业科技导报2025，Vol.27Issue(5)：133-145,13.DOI:10.13304/j.nykjdb.2023.0152
印度梨形孢介导小麦抗纹枯病的转录组分析及关键基因筛选
Transcriptome Analysis of Resistance to Sharp Eyespot of Wheat Mediated by Piriformospora indica and Key Genes Screening
摘要
Abstract
Sharp eyespot of wheat is a frequent soil borne disease worldwide,which has serious harm on stable and high yield of wheat.To clarify the induction effect of Piriformospora indica,an endophytic fungus,on wheat resistance to sharp eyespot and to use a biological method which breaking through the traditional chemical control mode to enhance wheat resistance to sharp eyespot,wheat seeds were soaked in P.indica spore solution,followed by inoculation with Rhizoctonia cerealis,and physiological and biochemical indicators and transcriptome results were analyzed.The results showed that pre-colonization of P.indica in wheat could enhance the activity of antioxidant enzymes,alleviate the reduction of relative water content caused by Rhizoctonia cerealis and increase the membrane stability that partially destroyed by the fungi by 39.6%,and could also raise the content of chlorophyll by 32.3%,improving the photosynthesis ability of wheat.The transcriptome analysis revealed that the colonization of P.indica could restore the transcriptome changes caused by pathogenic fungi,induce the expression of related resistance genes,and improved plant biomass by influencing hormonal metabolic pathways.The resistance of wheat to R.cerealis had been comprehensively promoted by various ways and means.Key genes including TraesCS1A02G372300 and TraesCS1B02G393100 etc had been screened based on the transcriptome data,which would provide theoretical and experimental basis for catching on the kernal layer of plant-microbe interaction and acceleration of the breeding work related to disease resistance and yield increase.关键词
印度梨形孢/小麦/纹枯病/转录组分析Key words
Piriformospora indica/wheat/sharp eyespot/transcriptome analysis分类
农业科技引用本文复制引用
史硕,冯宇,李亮,孟瑞,章延泽,杨秀荣..印度梨形孢介导小麦抗纹枯病的转录组分析及关键基因筛选[J].中国农业科技导报,2025,27(5):133-145,13.基金项目
国家自然科学基金项目(31801948) （31801948）
河北省省级科技计划项目(19226505D) （19226505D）
河北省自然科学基金项目(C2021202005). （C2021202005）
