中国比较医学杂志2025，Vol.35Issue(3)：30-39,47,11.DOI:10.3969/j.issn.1671-7856.2025.03.003
不同发育阶段BTBR T+Itpr3tf孤独症小鼠T细胞亚群异常研究
Abnormal T cell subsets of BTBR T+Itpr3tf autistic mice at different developmental stages
摘要
Abstract
Objective To investigate the expression of T cell subsets in the spleen of BTBR T+Itpr3tf autistic mouse at 4,8,and 12 weeks of age,and to determine the optimal age for studying the relationship between immune abnormalities and autism in BTBR autistic mice.Methods It randomly selected 5～6 male BTBR mouse at 4 weeks,8 weeks,and 12 weeks of age and C57BL/6J mouse of the same gender at corresponding ages for the three-box social interaction test,the self-grooming test,and the marble-burying test;Single cell suspensions were prepared from the spleens of mouse at 8 and 12 weeks of age,and flow cytometry was used to detect 8 subsets of T cells(TH 1,TH2,TH17,TC1,TC2,TC17,TFH,and Treg).Results Compared with C57BL/6J mouse of the same age,BTBR mouse at 4 weeks,8 weeks,and 12 weeks of age showed a decrease in social time(P＜0.001),an increase in grooming time(P＜0.01,P＜0.001),and an increase in the number of marbles buried(P＜0.01,P＜0.001)in BTBR mouse at 8 weeks and 12 weeks of age.As well,the expression of TH 1(P＜0.001),TH2(P＜0.01),TC 1(P＜0.05),TC2(P＜0.001),and TFH(P＜0.01)cells in 8-week-old BTBR mouse were significantly increased,while the expression of Treg(P＜0.001)cells were significantly decreased;The expression of TH 1(P＜0.01),TH2(P＜0.01),TH 17(P＜0.05),TC1(P＜0.01),TC2(P＜0.001),TC 17(P＜0.01),and TFH(P＜0.001)increased in 12-week-old BTBR mouse,while the expression of Treg(P＜0.05)cells decreased.At different age stages(P＜0.050)the ratio of TH 1/Treg and TC 1/Treg in 8-week-old BTBR mouse were significantly higher than those in 12 week old mouse,while the TC 17/Treg ratio decreased.Conclusions BTBR mouse at different developmental stages exhibit varying degrees of abnormal increase in Teff/Treg ratio.Based on result of behavioral test,it is recommended to use 8-week-old BTBR mice for research on autism and immune abnormalities.关键词
BTBR T+Itpr3tf小鼠/T细胞亚群/流式细胞术/孤独症Key words
BTBR T+Itpr3tf mice/T cell subset/flow cytometry/autism分类
医药卫生引用本文复制引用
申晨,李萌,聂祖庆,李志伟,文婕,杜娟娟,奎秀莹,杨菁瑞,曹霞..不同发育阶段BTBR T+Itpr3tf孤独症小鼠T细胞亚群异常研究[J].中国比较医学杂志,2025,35(3):30-39,47,11.基金项目
国家自然科学基金(82460281,82060306) （82460281,82060306）
云南省李斌专家工作站(202305AF150068) （202305AF150068）
云南省科技厅-昆明医科大学联合专项(202401AY070001-086). （202401AY070001-086）
