中国比较医学杂志2025，Vol.35Issue(3)：48-57,10.DOI:10.3969/j.issn.1671-7856.2025.03.005
两种造模方法建立弱精子症伴高SDF小鼠模型的比较研究
Comparative experimental study of sodium benzoate and corticosterone in a mouse model of asthenozoospermia with high sperm DNA fragmentation
摘要
Abstract
Objective To prepare a mouse model of asthenozoospermia(AZS)with high sperm DNA fragmentation(SDF)using corticosterone(CORT)and sodium benzoate(NaB).Methods Fifty 3-week-old male ICR mice were divided randomly into CORT-treated(n=30)and NaB-treated(n=20)groups.The CORT group was further divided into the following six groups(n=5 per group):high CORT(500 μg/mL),medium CORT(200 μg/mL),and low CORT(10 μg/mL)drinking water group,drinking water control group,CORT injection(40 mg/kg)group,and injection control group(normal saline).The animals were modeled continuously for 50 d.Mice in the NaB group were further divided into four groups(n=5 per group):high NaB(500 mg/kg),medium NaB(300 mg/kg),and low NaB(100 mg/kg)gavage groups,and control group(normal saline).The animals were modeled continuously for 50 d.The physiological state of the mice in each group was observed and mass changes were recorded continuously.The sperm motility capacity and DNA fragmentation index(DFI)of the sperm were observed from the tail of the epididymis after the end of the modeling.Results The rate of mass change in the CORT-injection moding group showed a downward trend.There was no significant difference(P＞0.05)in the high NaB gavage group,and the rate of body mass change in the high NaB gavage group was significantly decreased compared with the control group(P＜0.05).The percentages of forward motility sperm were significantly decreased in the CORT injection group(P＞0.05)and the percentage in the high NaB gavage group(P＜0.05),compared with the control group.The DFI was increased in the CORT injection group compared with the control group,but the difference was not significant(P＞0.05),and the DFI in the high NaB gavage group was significantly increased compared with the control group(P＜0.05).Conclusions Intragastric gavage with NaB 500 mg/(kg·d)for 50 d is an ideal method for constructing an animal model of AZS with high SDF.关键词
弱精子症伴高SDF/小鼠模型/苯甲酸钠/皮质酮Key words
asthenozoospermia with high SDF/mouse model/sodium benzoate/corticosterone分类
医药卫生引用本文复制引用
卢宗林,赵海洋,王晖,王鑫,孙自学..两种造模方法建立弱精子症伴高SDF小鼠模型的比较研究[J].中国比较医学杂志,2025,35(3):48-57,10.基金项目
国家自然科学基金(82274536),洛阳市科技发展计划项目(2022017Y). （82274536）
