中西医结合肝病杂志2025，Vol.35Issue(4)：430-437,8.DOI:10.3969/j.issn.1005-0264.2025.004.008
基于网络药理学与动物实验探究血府逐瘀汤治疗肝纤维化的作用机制
Exploring the mechanism of Xuefuzhuyu Decoction in treating liver fibrosis based on network pharmacology and animal experiments
摘要
Abstract
Objective:To explore the mechanism of Xuefuzhuyu Decoction in treating liver fibrosis through network pharmacology and animal experiments.Methods:The main components and potential targets of Xuefuzhuyu Decoction were obtained using TCMSP,ETCM,HERB,SwissTarget Prediction,and other databases.Liver fibrosis disease targets were retrieved from Genecard,OMIM,DRUGBANK,and TTD,and common targets between Xuefuzhuyu Decoction and liver fibrosis were identified.A protein interaction network was constructed to identify core targets.KEGG and GO enrichment analyses were conducted using Metascape to identify potential pathways.A mouse model of liver fibrosis was induced using CCl4,and liver tissue pathology,collagen type Ⅰ and Ⅲ expression,hepatic hydroxyproline(HYP)content,αSMA immunohistochemistry,and liver function changes were observed after Xuefuzhuyu Decoction intervention.RTqPCR was used to verify the changes in mRNA levels of key targets.Results:Xuefuzhuyu Decoction has 292 intersecting targets with liver fibrosis diseases,with core targets including AKT1,HIF-1α,mTOR,etc.KEGG enrichment analysis mainly involves cancer pathways,PI3K-AKT signaling pathway,HIF-1α signaling pathway,MAPK signaling pathway,etc.Animal experiments have shown that Xuefuzhuyu Decoction can improve liver fibrosis in mice,reduce the content of HYP and the expression of type Ⅰ and Ⅲ collagen in liver tissue,decrease the expression of α-SMA,alleviate liver inflammation,and downregulate the mRNA levels of PI3K,AKT1,mTOR,and HIF-1α in the liver.Conclusion:Xuefuzhuyu Decoction can improve liver fibrosis in mice,and its mechanism may be related to the regulation of the PI3K-AKT1-mTOR/HIF-1α pathway.关键词
血府逐瘀汤/肝纤维化/网络药理学Key words
Xuefuzhuyu Decoction/liver fibrosis/network pharmacology分类
医药卫生引用本文复制引用
谢金池,孟捷,苟思媛,毛堂友,王木源,何寅家,郑世豪,叶永安,张鹏..基于网络药理学与动物实验探究血府逐瘀汤治疗肝纤维化的作用机制[J].中西医结合肝病杂志,2025,35(4):430-437,8.基金项目
国家自然科学基金项目(No.82104810),河北省中医药管理局科研计划项目(No.B2025045),北京中医药大学东方医院高水平能力建设项目(No.DFGZRB-2024GJRC015) （No.82104810）
