中西医结合肝病杂志2025，Vol.35Issue(4)：438-443,6.DOI:10.3969/j.issn.1005-0264.2025.004.009
茯苓多糖调控AMPK信号通路干预脂质代谢改善非酒精性脂肪性肝病的机制研究
The mechanism of Pachymaran ameliorating nonalcoholic fatty liver disease by regulating AMPK signaling pathway and lipid metabolism
摘要
Abstract
Objective:To investigate the effect and mechanism of Pachymaran on nonalcoholic fatty liver disease(NAFLD)induced by high-fat diet.Methods:Thirty C57BL/6 mice were divided into normal diet,NAFLD,high-,medium-and low-dose pachymaran groups,with 6 mice in each group.Except the normal diet group,the other groups were fed high-fat diet to induce NAFLD.Pachymaran groups irrigation to 1 time a day,eight weeks in a row.Then gather the liver,serum level of each index,and HE staining to observe the liver pathology.Western blotting test amp activated protein kinase(AMPK)expression,RT-qPCR detection of triglyceride metabolism enzyme mRNA level.Results:The body weight and body fat of the high Pachymaran dose group decreased significantly after treatment and serum lipid related indicators decreased significantly(P＜0.05).Tissue staining showed hepatocyte hypertrophy accompanied by cystic steatosis and increased hepatocyte lipid droplets in NAFLD group,and the above conditions were significantly reduced after treatment(P＜0.05).Western blotting revealed that AMPK phosphorylation level significantly increased after the treatment,and RT-qPCR results triglyceride synthetase significantly reduced,degrading enzymes rise significantly(P＜0.05).Conclusion:Pachymaran inhibits fat accumulation,hepatocyte degeneration,and damage in NAFLD mice.Its mechanism may involve the regulation of the AMPK pathway and improvement in triglyceride metabolism,thus modulating the pathological progression of NAFLD.关键词
茯苓多糖/非酒精性脂肪肝/腺苷酸活化蛋白激酶/肝脏脂肪Key words
Pachymara/nonalcoholic fatty liver disease/amp activated protein kinase/liver fat分类
医药卫生引用本文复制引用
禹红,刘星,张凡雄,李红,李耕..茯苓多糖调控AMPK信号通路干预脂质代谢改善非酒精性脂肪性肝病的机制研究[J].中西医结合肝病杂志,2025,35(4):438-443,6.基金项目
湖北省中医药管理局中医药科研项目(No.ZY2023F052、ZY2023F005),武汉市卫健委中医药科研项目(No.WZ24A08) （No.ZY2023F052、ZY2023F005）
