中西医结合肝病杂志2025，Vol.35Issue(4)：444-450,7.DOI:10.3969/j.issn.1005-0264.2025.004.010
胆宁片与益生菌对大鼠非酒精性脂肪性肝病的治疗作用及其机制研究
The therapeutic effects and mechanisms of Danning Tablets and probiotics on non-alcoholic fatty liver disease in rats
摘要
Abstract
Objective:The study aimed to assess the therapeutic effects of Daning tablets and probiotics on non-alcoholic fatty liver disease(NAFLD)in rats,as well as their influence on the TLR4/NF-κBP65/MyD88/P38 signaling pathway in the liver.Methods:Forty male Sprague-Dawley rats were randomly assigned to a control group(n=10)fed a standard diet,and a NAFLD model group(n=30)fed a high-fat diet for 8 weeks to induce NAFLD.Following successful modeling,the NAFLD model group was subdivided into a model group(n=10),treatment 1 group(n=10,treated with Daning tablets),and treatment 2 group(n=10,treated with probiotics).Following an 8-week treatment period,the rats were euthanized and samples of peripheral blood and liver tissue were obtained.Subsequently,levels of endotoxin lipopolysaccharide(LPS),tumor necrosis factor α(TNF-α),interleukin-1 β(IL-1β),interleukin-6(IL-6),total cholesterol(TC),triglycerides(TG),alanine aminotransferase(ALT),aspartate transaminase(AST),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),superoxide dismutase(SOD),and malondialdehyde(MDA)were quantified.HE staining was utilized for morphological analysis of liver tissues in each experimental group.Immunohistochemistry and Western blotting techniques were employed to assess the expression levels of Toll-like receptor 4(TLR4),myeloid differentiation factor 88(MyD88),nuclear factor κB P65(NF-κB P65),P38 mitogen-activated protein kinase(P38 MAPK),phosphorylated P38 mitogen-activated protein kinase(P-P38),c-Jun N-terminal kinase(JNK),and caspase-1 in the liver tissues.Results:The TC,TG,ALT,AST,LDL-C,LPS,and MDA in the NAFLD model group were found to be significantly elevated compared to those in the control group.Conversely,HDL-C and SOD levels were significantly lower in the NAFLD model group.Additionally,the expression levels of TLR4,MyD88,NF-κB P65,P38 MAPK,P-P38,c-JUN,and Caspase-1 proteins in liver tissues were higher in the NAFLD model group compared to the control group.Treatment with groups 1 and 2 resulted in significantly decreased levels of TC,TG,ALT,AST,LDL-C,LPS,MDA,as well as reduced liver TC,TG,and free fatty acid(FFA)content compared to the model group.Conversely,SOD and HDL-C levels were significantly increased in the treatment groups.The levels of ALT,AST,LPS,and MDA in Treatment Group 2 were significantly lower than those in Treatment Group 1,while SOD and HDL-C levels were significantly increased.Liver TLR-4,MyD88,NF-κBP65,P38MAPK,P-P38,and Caspase 1 protein expression in Treatment Groups 1 and 2 were significantly lower than in the model group,with Treatment Group 2 showing lower levels compared to Treatment Group 1.Conclusion:The concurrent administration of Daning tablets and Bifidobacterium has been shown to effectively ameliorate hepatic function impairment in NAFLD rats by attenuating oxidative stress,reducing inflammatory cytokine levels,and potentially inhibiting TR4/NF-κBP65/My D88 activation,thereby exerting protective effects on the liver in NAFLD.关键词
非酒精性脂肪肝性肝病/胆宁片/布拉氏酵母菌散/Toll样受体-4/肿瘤坏死因子αKey words
non-alcoholic fatty liver disease/Danning tablets/bifidobacterium bifidum powder/toll-like receptor-4/tumor necrosis factor α分类
医药卫生引用本文复制引用
王富兵,赵培培,范辉,顾新红,李欣,徐霞,张谷..胆宁片与益生菌对大鼠非酒精性脂肪性肝病的治疗作用及其机制研究[J].中西医结合肝病杂志,2025,35(4):444-450,7.基金项目
南通市基础科学研究和社会民生科技计划项目(No.MSZ2022135) （No.MSZ2022135）
