植物营养与肥料学报2025，Vol.31Issue(4)：718-729,12.DOI:10.11674/zwyf.2024431
褪黑素通过调控根系形态生理特性增强大豆幼苗对抗低磷胁迫的能力
Melatonin enhances the resistance of soybean seedlings to low-phosphorus stress by regulating root morphological and physiological characteristics
摘要
Abstract
[Objectives]We analyzed the key indicators for melatonin to promote the resistance of soybean seedlings to low phosphorus stress,from the perspective of regulating root morphology and physiological characteristics.[Methods]Soybean seedlings at 1 compound leaf stage(V1 stage)were used as materials to carry out a sand culture experiment.The P concentration 0.5 and 0.05 mmol/L were set up as normal phosphorus control(CK)and low P stress(P0.05),and applying 100 μmol/L of melatonin under low P stress was taken as melatonin treatment(P0.05+M).At the 0,5,10,15,and 20 days after the melatonin treatment,soybean plants and roots were sampled for investigation of plant growth,root morphological indicators,and the determination of physiological indicators.[Results]Compared with CK,P0.05 treatment significantly inhibited the growth of soybean seedlings.Compared with the P0.05 treatment,at days 5,10,15,and 20,the P0.05+M treatment increased the activities of glutamine synthetase(GS),glutamate synthetase(GOGAT),glutamate dehydrogenase(GDH),glutamate oxaloacetate transferase(GOT),glutamate pyruvate transaminase(GPT),and the contents of soluble protein and free amino acids in roots by an average of 10.63%,4.3%,4.8%,13.73%,6.18%,7.05%,5.23%,but decreased ammonium and nitrate nitrogen content by 9.7%and 5.41%,respectively.On the 10th and 20th days,the P0.05+M treatment increased soybean root length,root volume,number of root nodules,root surface area,average diameter,number of root tip,and root vitality by an average of 4.59%,27.51%,12.67%,7.27%,3.57%,22.23%,and 11%compared to the P0.05 treatment;the average nitrogen and phosphorus accumulation of leaves,stems,roots,and the whole plant increased by 108.37%,201.82%,58.93%,87.38%,and 62.69%,63.24%,55.84%,and 59.96%,respectively;the content of photosynthetic pigments Chla,Chlb,Chl(a+b),Car and gas exchange parameters Pn,Gs,Ci,Tr increased by an average of 13.02%,23.02%,15.66%,29.59%and 16.64%,10.4%,3.2%,13.04%,respectively;the average increase in plant height,stem thickness,leaf area,number of nodes,leaf dry weight,stem dry weight,root dry weight,and total plant dry matter accumulation was 22.88%,17.09%,62.07%,20.97%,52.43%,25.6%,33.93%,and 38.58%,respectively.The Mantel test results showed that there was a positive correlation between most root morphological and physiological indicators,as well as between them and the aboveground dry matter,nitrogen,and phosphorus accumulation.Random forest analysis further clarified that root nitrate nitrogen content,glutamate pyruvate transaminase activity,root surface area,and ammonium nitrogen content were the four most critical indicators determining the growth rate of soybean seedlings under low phosphorus stress.[Conclusions]The most important indicators directly influencing the growth rate of soybean seedlings are root nitrate nitrogen content,glutamate pyruvate transaminase activity,root surface area,and ammonium nitrogen content.Melatonin application significantly improve root development and the activities of glutamate pyruvate transaminase,stimulate the up-translocation of nitrogen and phosphorus in roots,thus alleviate the low-phosphorus stress.关键词
大豆/低磷胁迫/褪黑素/根系形态和生理/幼苗生长Key words
soybean/low phosphorus stress/melatonin/root morphology and physiology/seedling growth引用本文复制引用
吴天一,贝世俊,褚霈宇,吴耀坤,贾鸿昌,朱嘉宇,商晓寒,王孟雪,金喜军..褪黑素通过调控根系形态生理特性增强大豆幼苗对抗低磷胁迫的能力[J].植物营养与肥料学报,2025,31(4):718-729,12.基金项目
国家重点研发计划项目(2022YFD1000105) （2022YFD1000105）
黑龙江八一农垦大学研究生创新科研项目(YJSCX2023-ZX03) （YJSCX2023-ZX03）
黑龙江省"揭榜挂帅"科技攻关项目(2021ZXJ05B011) （2021ZXJ05B011）
黑龙江省省属科研院所科研业务费项目(CZKYF2023-1-B016). （CZKYF2023-1-B016）
