安徽农业科学2025，Vol.53Issue(9)：65-68,71,5.DOI:10.3969/j.issn.0517-6611.2025.09.015
牛源大肠杆菌多重耐药机制研究
Study on the Mechanism of Multi-drug Resistance of Bovine Escherichia coli
摘要
Abstract
[Objective]To understand the drug resistance and integron and soxS gene mutations of bovine Escherichia coli in farms in Hei-longjiang Province.[Method]Antimicrobial susceptibility experiments were carried out by K-B disc method,and the mechanism of multi-drug resistance was studied by PCR method.[Result]Escherichia coli from both beef cattle and dairy cattle showed multi-drug resistance.Escherichia coli from dairy cows had the highest resistance rate to streptomycin,which was 48.75%.Beef cattle Escherichia coli had the highest resistance rate to tetracycline,which was 66.67%.Integron Ⅰ was detected in beef cattle with a gene cassette type of dfrA17.Integron Ⅱ was detected in dairy cows,and the gene box type was dfrA1.The detection rate of soxS gene was 44.52%,and sequence analysis showed that one dairy cow source had an amino acid sequence site mutation Ala12 → Ser.[Conclusion]This study provides scientific reference for guiding clinical rational drug use and the development of new drugs to curb bacterial efflux pump mechanism in the process of cattle breeding.关键词
牛/大肠杆菌/多重耐药性/机制/整合子-基因盒/soxS基因Key words
Cattle/Escherichia coli/Multi-drug resistance/Mechanism/Integron-gene cassette/soxS gene分类
农业科技引用本文复制引用
杨海旺,邵莉涵,田瑞瑶,孟翔,霍亚迪,赵换丽,刘思梦,薛原..牛源大肠杆菌多重耐药机制研究[J].安徽农业科学,2025,53(9):65-68,71,5.基金项目
大学生创新创业训练计划项目(202410225588) （202410225588）
黑龙江省博士后科研启动项目(LBH-Q14002). （LBH-Q14002）
