生物资源2025，Vol.47Issue(2)：118-128,11.DOI:10.14188/j.ajsh.20250104001
湖北南河国家级自然保护区虫生真菌资源调查及物种多样性研究
Investigation and species diversity of entomogenous fungi in Nanhe National Nature Reserve,Hubei
摘要
Abstract
Entomogenous fungi are important members of biological species diversity and ecosystem.In order to in-vestigate the species diversity of entomogenous fungi in Nanhe National Nature Reserve,Hubei,8 field surveys were car-ried out in this reserve from 2023 to 2024.The pure culture strains were obtained by tissue isolation method or spread plate method,and DNA was extracted.Morphological characteristics and internal transcribed spacer(ITS)-based mo-lecular phylogenetic analysis showed that a total of 83 samples of entomogenous fungi were identified as 3 families,6 genera and 14 species,including Metarhizium anisopliae in Clavicipitaceae,Ophiocordyceps nutans and Purpureocillium taka-mizusanense in Ophiocordycipitaceae,and Beauveria bassiana,B.pseudobassiana,Cordyceps cateniannulata,C.fumos-orosea,C.javanica,C.lepidopterorum,C.militaris,C.pruinosa,C.tenuipes,Samsoniella aurantia and Samsoniella spp.in Cordycipitaceae.The dominant taxa were Cordycipitaceae,Cordyceps,Cordyceps tenuipes and C.lepidoptero-rum.Purpureocillium takamizusanense and Samsoniella aurantia were newly recorded species in Hubei.The findings in-crease our understanding of the diversity of entomogenous fungi in Nanhe National Nature Reserve,Hubei,enrich the flo-ra distribution of entomogenous fungi in China,and provide germplasm resources for the conservation and utilization of this fungal group.关键词
虫生真菌/白僵菌属/虫草属/绿僵菌属/线虫草属/紫孢霉属/鳞翅虫草属Key words
entomogenous fungi/Beauveria/Cordyceps/Metarhizium/Ophiocordyceps/Purpureocillium/Sam-soniella分类
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李志愿,李武燕,黄呈鑫,牟枭,姚芬,赵珂欣,邓正群,余知和..湖北南河国家级自然保护区虫生真菌资源调查及物种多样性研究[J].生物资源,2025,47(2):118-128,11.基金项目
湖北南河国家级自然保护区2022年中央财政林业草原生态保护恢复资金项目(PAXG-DL-2023012) （PAXG-DL-2023012）
