现代中医临床2025，Vol.32Issue(3)：6-13,8.DOI:10.3969/j.issn.2095-6606.2025.03.002
基于蛋白质组学探讨清肝健脾法治疗桥本甲状腺炎的作用机制
Exploring the mechanism of the liver-clearing and spleen-strengthening methods in the treatment of Hashimoto's thyroiditis based on proteomics
摘要
Abstract
Objective To explore the potential therapeutic targets of liver-clearing and spleen-strengthening method in the treatment of Hashimoto's thyroiditis(HT)using proteomic method.Methods A total of 54 patients with HT were included and divided 1:1 into a TCM group and a control group.The control group was given a low-iodine diet,while the TCM group was given additionally liver-clearing and spleen-strengthening formulas orally on this basis,with an observation period of 4 weeks.Ten patients were randomly selected for serum samples before and after treatment,and the serum proteins were identified and quantitatively analyzed using TMT proteomic technology to screen for differential proteins before and after treatment.Bioinformatics analysis was conducted for protein functional annotation,enrichment analysis,and protein-protein interaction analysis.For the remaining 44 subjects,serum samples before and after treatment were used to detect the expression levels of candidate differential proteins by Elisa,verifying the result of proteomic studies.Results After 4 weeks of treatment,the TPOAb in the traditional Chinese medicine group decreased significantly compared to before treatment(P=0.004),while the TPOAb in the control group increased compared to before treatment.A total of 653 proteins were identified in the proteomic study,and 58 potential differential proteins related to the efficacy of liver-clearing and spleen-strengthening formula were screened.Protein functional annotation revealed that the candidate differential proteins were mainly involved in inflammatory responses,immune responses,cell and tissue structure formation,intercellular signal transduction,oxidative stress,and apoptosis.Elisa analysis selected three differential proteins related to the pathogenesis of HT,APOE,SAP,and S100A9,and the result showed that the levels of them were significantly decreased after treatment.Conclusion The liver-clearing and spleen-strengthening method can effectively reduce the level of autoantibodies in HT patients,and inflammatory factors such as APOE,SAP,and S100A9 may be important targets,exerting therapeutic effects by regulating immune inflammatory responses.关键词
清肝健脾法/桥本甲状腺炎/蛋白质组学/免疫炎症反应Key words
the liver-clearing and spleen-strengthening methods/Hashimoto's thyroiditis/proteomics/immune-inflammatory response分类
中医学引用本文复制引用
袁姣姣,耿文倩,吴艳珂,娄文凤,丁治国..基于蛋白质组学探讨清肝健脾法治疗桥本甲状腺炎的作用机制[J].现代中医临床,2025,32(3):6-13,8.基金项目
国家科技部外国专家项目(No.G2023110002L) （No.G2023110002L）
陕西省卫生健康委员会-陕西省卫生健康高层次人才(团队)培育计划项目(No.陕卫人函[2024]192号) （团队）
陕西省中医药管理局项目(No.SZY-NLTL-2024-031) （No.SZY-NLTL-2024-031）
