现代中医临床2025，Vol.32Issue(3)：14-19,6.DOI:10.3969/j.issn.2095-6606.2025.03.003
基于流式微球阵列术探索清肝散结消瘿方的免疫调节作用
Exploring the immunomodulatory effects of Qinggan Sanjie Xiaoying Formula based on cytometric bead array technology
摘要
Abstract
Objective To explore cytokine profile changes in patients with Hashimoto's thyroiditis(HT)and the immunomodulatory effects of Qinggan Sanjie Xiaoying Formula(QGSXYF)using cytometric bead array(CBA)technology.Methods A total of 28 patients with HT,who sought treatment at Sun Simiao Hospital of Beijing University of Chinese Medicine and Dongzhimen Hospital of Beijing University of Chinese Medicine from September 2022 to December 2023 and voluntarily received treatment with QGSXYF,were enrolled as the HT group.Meanwhile,20 healthy volunteers were recruited as the healthy control group.Serum samples were collected from HT patients before and after four weeks of treatment with QGSXYF.Thyroid antibody titers(TPOAb,TGAb)and cytokine profile changes were compared among the three groups.Results In the healthy control group,TPOAb and TGAb were within the normal range and were significantly lower than those in HT patients(P<0.01).After treatment with QGSXYF,HT patients showed a significant reduction in TPOAb titers compared to pre-treatment levels(P<0.01).Compared with the healthy control group,multiple cytokine levels in HT patients were significantly elevated(P<0.05).Following treatment with QGSXYF,serum levels of IL-6 and IL-8 in HT patients decreased significantly(P<0.01),while other pro-inflammatory cytokines such as IL-1β,IL-2,IL-5,IL-17A,IL-17F,IL-22,TNF-α,and IFN-γ also showed reductions(P<0.05).Additionally,the levels of anti-inflammatory cytokines IL-4 and IL-10 increased significantly after treatment with the herbal remedies(P<0.05).Conclusion Cytokine levels in HT patients were significantly higher than those in the healthy control group.QGSXYF effectively reduced TPOAb titers by significantly lowering pro-inflammatory cytokines(e.g.,IL-6,IL-8,IL-22)and increasing anti-inflammatory cytokines(IL-4,IL-10),thereby modulating Th1/Th2 balance and Th17/Treg cell function.These findings suggest that the immunoregulatory effects of this formula may be related to multi-targeted intervention in inflammatory pathways.关键词
桥本甲状腺炎/细胞因子/流式微球阵列术/清肝散结消瘿方/免疫Key words
Hashimoto's thyroiditis/cytokines/cytometric bead array/Qinggan Sanjie Xiaoying Formula/immunity分类
中医学引用本文复制引用
娄文凤,丁治国,周询,李心爱,祁烁..基于流式微球阵列术探索清肝散结消瘿方的免疫调节作用[J].现代中医临床,2025,32(3):14-19,6.基金项目
陕西省中医药"双链融合"中青年科研创新团队建设项目(No.2022-SLRH-LG-005) （No.2022-SLRH-LG-005）
陕西省"秦创原"中医药创新研发转化项目(No.2022-QCYZH-043) （No.2022-QCYZH-043）
铜川市甲状腺病防治中心项目(No.TJF-2022-18,No.TJF-MS-2023-01) （No.TJF-2022-18,No.TJF-MS-2023-01）
