现代中医临床2025，Vol.32Issue(3)：39-43,5.DOI:10.3969/j.issn.2095-6606.2025.03.008
基于TGF-β1/CTGF信号通路探讨艾灸对压力性尿失禁大鼠的作用及机制
Efficacy and mechanism of moxibustion for managing stress urinary incontinence in rats from the perspective of activating TGF-β1/CTGF signal pathways
摘要
Abstract
Objective To study the effecacy and mechanism of moxibustion on stress urinary incontinence(SUI)in rats.Methods SUI rat model was established by bilateral ovariectomy combined with vaginal dilatation.Forty SD rats were randomly divided into 3 groups according to the random number table:The moxibustion intervention group(Mox)(n=15)was treated with moxibustion,the control group(NC)(n=15)was only treated with bilateral oophorectomy combined with vaginal dilatation,and the blank group(n=10)did not undergo any interventions.The leak point pressure(LPP)was measured,and Sirius red staining of urethral tissue and Van Gieson staining(EVG),real-time fluorescent quantitative PCR(qRT-PCR)were used to detect the mRNA levels of collagen Ⅰ,Ⅲand α-actin(α-SAM),and Western blot was used to detect the expression of TGF-β1 and CTGF to evaluate the therapeutic effect and molecular mechanism of moxibustion on urinary incontinence.Results Compared with the blank group,the LPP of the NC group and the Mox group decreased significantly(P<0.01),the Sirius red staining and EVG staining showed that the positive area of collagen and elastic fibers decreased(P<0.01),and the mRNA levels of collagen Ⅰ,collagen Ⅲ and α-SAM decreased significantly(P<0.01).The protein expression of TGF-β1 and CTGF was down-regulated.Compared with the control group,the LPP,the proportion of collagen and elastic fibers in the urethral tissue,the mRNA levels of collagen Ⅰ,collagen Ⅲ and α-SAM,and the protein expression of TGF-β1 and CTGF were significantly increased in the Mox group(P<0.01).Conclusion Moxibustion can improve the urination function of SUI rats and reduce urethral injury,which may relieve urinary incontinence by activating TGF-β1/CTGF pathway to restore collagen and elastic fiber structure in urethral tissue.关键词
压力性尿失禁/艾灸/细胞外基质/胶原蛋白/弹性纤维Key words
stress urinary incontinence/moxibustion/extracellular matrix/collagen/elastic fiber分类
医药卫生引用本文复制引用
刘冬,马小娜,张晨,秦丽娜,吕桃桃,康春嵋..基于TGF-β1/CTGF信号通路探讨艾灸对压力性尿失禁大鼠的作用及机制[J].现代中医临床,2025,32(3):39-43,5.基金项目
国家重点研发计划项目(No.2022YFC2503900,No.2022YFC2503902) （No.2022YFC2503900,No.2022YFC2503902）
