表面技术2025，Vol.54Issue(10)：225-236,12.DOI:10.16490/j.cnki.issn.1001-3660.2025.10.018
水热及化学处理对烧结羟基磷灰石生物活性的影响
Effect of Hydrothermal and Chemical Treatment on Bioactivity of Sintered Hydroxyapatite
摘要
Abstract
Sintered hydroxyapatite(S-HAp)has great potential for applications in bone repair,but its insufficient cellular bioactivity limits the use.To improve the cellular bioactivity of S-HAp,this study investigated the effects of three surface treatment methods-hydrothermal,sodium hydroxide(NaOH)boiling,and sodium fluoride(NaF)soaking-along with various process parameters such as hydrothermal temperature and NaOH/NaF concentration on the proliferation activity of osteoblasts on S-HAp surfaces.The mechanisms were revealed based on the surface morphology,elemental composition,wettability,pH,and other characteristics of S-HAp before and after treatment.The results indicated that hydrothermal treatment could create a large number of loose and porous structures on the S-HAp surface,enhancing its hydrophilicity and thus improving cellular proliferation activity;moreover,higher hydrothermal temperatures led to great pronounced improvement in cellular proliferation.The 1,3 and 7 d values of OD 450 of S-HAp treated by hydrothermal at 240℃were almost 50%higher than that of the control group.NaOH treatment could deposit NaOH crystals on the S-HAp surface,neutralizing the acidic substances produced during cell growth and proliferation,allowing the cell culture medium to maintain a weakly alkaline environment for a longer time and thereby enhancing cellular proliferation activity.The 0.4 mol/l NaOH treatment improved the 7 d OD 450 value of S-HAp by 75%compared with the control group.However,excessively high NaOH concentration could lead to an overly alkaline medium,inhibiting initial cell growth and proliferation.The 1 d OD 450 values of S-HAp treated by 0.8 and 1.6 mol/L NaOH were both lower than that of the control group.As time went on,the acidic substances produced during cell growth and proliferation continually accumulated,leading the pH to a suitable range,and then the OD 450 values of S-HAp treated by 0.8 and 1.6 mol/L NaOH exceeded that of the control group.Additionally,NaF could chemically react with S-HAp,introducing fluoride ions on the S-HAp surface to stimulate cell growth and proliferation,improving cellular proliferation activity.However,excessive fluoride ion concentrations could be toxic to the cells.During the experimental range,the S-HAp samples soaked by NaF at various concentrations all obtained larger OD 450 values than the control group,but the values continuously decreased as the NaF concentration increased.The three treatment methods,hydrothermal,NaOH boiling,and NaF soaking,all can enhance the cell proliferation activity of sintered hydroxyapatite.By comparing the effects of different treatments on the proliferation activity of S-HAp samples,it can be seen that the 0.4 mol/L NaOH treatment obtained the best improvement effect in the experimental range.However,the pH regulation function of sodium hydroxide can be affected by the content and flow rate of tissue fluid around the implant,and the effect is poorly controllable.Therefore,hydrothermal and sodium fluoride treatment are more suitable for improving the cell proliferation activity of sintered hydroxyapatite implants.But it should be noted that the effect of hydrothermal treatment on the strength and wear-resisting property of implants should be further studied,while the poisonousness of implants with sodium fluoride treatment must be tightly verified one by one before clinical application.关键词
烧结羟基磷灰石/细胞增殖/水热处理/氢氧化钠处理/氟化钠处理/润湿性Key words
sintered hydroxyapatite/cell proliferation/hydrothermal/NaOH treatment/NaF soaking/wettability分类
矿业与冶金引用本文复制引用
刘国梁,李程程,张凤,邢宏宇,孙树峰,冀敏..水热及化学处理对烧结羟基磷灰石生物活性的影响[J].表面技术,2025,54(10):225-236,12.基金项目
国家自然科学基金资助项目(52375446,52305478) （52375446,52305478）
山东省自然科学基金资助项目(ZR2023QE178)National Nature Science Foundation of China(52375446,52305478) （ZR2023QE178）
Natural Science Foundation of Shandong Province(ZR2023QE178) （ZR2023QE178）
