草业学报2025，Vol.34Issue(5)：12-26,15.DOI:10.11686/cyxb2024246
降水变化条件下荒漠草原优势植物根际微生物群落结构和多样性特征研究
Structure and diversity characteristics of the rhizosphere microbial community of dominant plants on the desert steppe under changing precipitation
摘要
Abstract
The aim of this study was to explore the responses of rhizosphere soil microorganisms of dominant plant species on the Ningxia desert steppe to changes in precipitation.The overall aim was to broaden our understanding of the interactions between microorganisms and the roots of important plants on the desert steppe.Three plant species were selected for analysis of their rhizobial communities:Stipa breviflora,Lespedeza potaninii,and Convolvulus ammannii.Five rainfall treatments were established:50%increase in rainfall,30%increase in rainfall,control group,30%decrease in rainfall,and 50%decrease in rainfall.High-throughput sequencing data were analyzed to determine differences in the structure and diversity of the rhizosphere microbial communities of the three dominant species on the desert steppe under different precipitation treatments.The results showed that the aboveground biomass of dominant plant species on the desert steppe increased with increasing precipitation,and was significantly positively correlated with the soil water content(P<0.05).In the rhizosphere soil of the three dominant plants,the dominant bacterial phyla were Actinomycetes,Proteobacteria,Acidobacteria,and Bacteroidetes.The dominant phyla in the fungal community were Ascomycetes and Basidiomycetes.In all the treatments,the rhizosphere bacterial communities were more sensitive than the fungal communities to the water level.The maximum numbers of fungal and bacterial operational taxonomic units,and the highest microbial activity in rhizosphere soil,were in the 30%decreased rainfall treatment.The relative abundance of Cyanobacteria and Bacteroidetes in the rhizosphere bacterial community was higher in C.ammannii than in L.potaninii and S.breviflora,while the relative abundance of Planctomycetota and Acidobacteriota in the rhizosphere bacterial community was lower in C.ammannii than in than in L.potaninii and S.breviflora.The rhizosphere microbial communities were similar in L.potaninii and S.breviflora,but that of C.ammannii was different.The diversity of the bacterial community in the rhizosphere of S.breviflora was significantly negatively correlated with total phosphorus(P<0.05).The diversity of the bacterial community in the rhizosphere of C.ammannii was significantly positively correlated with total potassium(P<0.05),However,the diversity of the total microbial community in the rhizosphere of S.breviflora was significantly negatively correlated with total potassium(P<0.05).Soil pH was significantly positively correlated with the diversity of the rhizosphere soil bacterial community(P<0.05),and negatively correlated with the diversity of the rhizosphere soil fungal microbial community(P<0.05).There was a significant correlation between the rhizosphere soil microbial communities of L.potaninii and S.breviflora,and there was a certain complementary symbiotic effect.关键词
荒漠草原/优势植物/降水变化/高通量测序/根际微生物群落Key words
desert steppe/dominant plants/precipitation variability/high-throughput sequencing/rhizosphere microbial community引用本文复制引用
邓文辉,宋珂辰,张浩,管思雨,雍嘉仪,胡海英..降水变化条件下荒漠草原优势植物根际微生物群落结构和多样性特征研究[J].草业学报,2025,34(5):12-26,15.基金项目
国家自然科学基金项目(32160406)和宁夏高等学校一流学科建设(草学学科)项目(NXYLXK2017A01)资助. （32160406）
