草业学报2025，Vol.34Issue(5)：74-88,15.DOI:10.11686/cyxb2024257
结缕草DREB基因家族的鉴定及非生物胁迫下的表达模式分析
Identification of DREB genes from Zoysia japonica and their transcript profiles in response to abiotic stress
摘要
Abstract
DREB transcription factors belong to the AP2/ERF superfamily,and play important roles in plant development and stress responses.However,a systematic analysis of DREB genes in Zoysia japonica,which is a warm-season turfgrass native to China has not been reported.In this study,we identified 64 ZjDREBs from Z.japonica,encoding polypeptides of 105 to 984 amino acids,with molecular weights ranging from 11.42 to 107.04 kDa and theoretical isoelectric points ranging from 4.09 to 11.95.The phylogenetic analysis of 64 ZjDREBs showed that they were divided into six groups from A1 to A6.A conserved motif analysis of ZjDREB protein sequences revealed that motif1,motif2,and motif3 were relatively highly conserved.Sixty-one ZjDREBs were unevenly distributed on 14 chromosomes of Z.japonica.By colinearity analysis,we identified three pairs of segmentally duplicated ZjDREB genes.The non-synonymous∶synonymous(Ka/Ks)was<1,indicating that this gene family might have been subject to purifying selection during its expansion.Analyses of the promoter regions of ZjDREBs in the A1 and A2 groups revealed multiple cis-acting elements related to plant hormones and abiotic stresses.The expression profiles of ZjDREB genes in the A1 and A2 groups under cold,abscisic acid(ABA),drought,and salt stress were detected by quantitative real-time polymerase chain reaction(qRT-PCR).The A1 group genes were up-regulated by cold and ABA treatments,and ZjDREB3 was significantly up-regulated.The A2 group genes showed different expression profiles under drought and salt stress,and ZjDREB4 was significantly up-regulated.Together,these results lay a foundation for further exploration of the functions of ZjDREB genes and provide avenues for the genetic improvement of Z.japonica.关键词
结缕草/DREB转录因子/系统进化/非生物胁迫/表达模式Key words
Zoysia japonica/DREB transcription factor/phylogenetic evolution/abiotic stress/expression profiling引用本文复制引用
左志芳,李永龙,魏雨佳,周生辉,李岩,杨国锋..结缕草DREB基因家族的鉴定及非生物胁迫下的表达模式分析[J].草业学报,2025,34(5):74-88,15.基金项目
山东省牧草产业技术体系(SDAIT-23-01)资助. （SDAIT-23-01）
