草业学报2025，Vol.34Issue(5)：105-117,13.DOI:10.11686/cyxb2024258
基于非靶向代谢组学分析丛枝病感病与未感病苦豆子代谢物的差异
Analysis of metabolite differences in Sophora alopecuroides infected and non-infected by witches'broom disease based on non-targeted metabolomics
摘要
Abstract
Phytoplasma is a bacterial pathogen that induces a range of symptoms in host plants,including yellowing,apical necrosis,witches'broom,phyllody,dwarfism,and floral changes.In China,more than 100 types of phytoplasma diseases have been identified that affect important economic crops,traditional Chinese medicinal herbs,and forestry.Witches'broom disease,caused by phytoplasma infection in Sophora alopecuroides,inhibits growth,causes seed production failure,and reduces yield,posing a potential threat to industries that utilize S.alopecuroides in China.This study focused on the impact of phytoplasma infection on S.alopecuroides metabolites by comparing infected and healthy plants using morphological,molecular biological,and untargeted metabolomic analyses.Our results indicate that the phytoplasma strain causing witches'broom disease in S.alopecuroides belongs to the 16Sr V-B subgroup.Infection significantly altered the metabolite profile of S.alopecuroides,with differentially annotated metabolites primarily associated with environmental information processing,genetic information processing,and metabolism,with the latter constituting 92.38%of total observed metabolite changes.KEGG pathway analysis revealed significant differences in the porphyrin and chlorophyll metabolism pathways as well as in the biosynthesis of phenylalanine,tyrosine,and tryptophan.It is speculated that phytoplasma infection may induce disease symptoms in the host plant primarily through changes in host metabolites.Alterations in these two pathways play a crucial role in the occurrence of witches'broom disease in S.alopecuroides,suggesting that infection reduces the photosynthetic capacity of the host,leading to nutrient deficiency and decreased resistance,which in turn aids disease progression.关键词
苦豆子/丛枝病/非靶向代谢组学/KEGG分析Key words
Sophora alopecuroides/witches'broom disease/non-targeted metabolomics/KEGG analysis引用本文复制引用
赛牙热木·哈力甫,杨莉,李冠宏,朱永琪,李东..基于非靶向代谢组学分析丛枝病感病与未感病苦豆子代谢物的差异[J].草业学报,2025,34(5):105-117,13.基金项目
石河子大学高层次人才科研启动项目(RCZK2021B23)和石河子大学自主资助支持专项(ZZZC2022009)资助. （RCZK2021B23）
