草业学报2025，Vol.34Issue(5)：118-129,12.DOI:10.11686/cyxb2024255
青稞根腐病根际土壤细菌多样性及群落结构变化规律
Bacterial diversity and community structural changes in rhizosphere soil of naked barley disturbed by root rot
摘要
Abstract
The occurrence and spread of naked barley(Hordeum vulgare var.nudum)root rot are known to be closely related to the dynamics of the rhizosphere soil bacterial community.This study systematically investigated naked barley root rot in fields of the Tibetan Gannan Autonomous Prefecture,Gansu Province,to clarify the influences on the diversity and structure of the rhizosphere soil bacterial community disturbed by naked barley root rot.Rhizosphere soil samples with different root rot incidences were collected,and genetic characterization of the bacterial community was obtained by high-throughput sequencing.The structural dynamics and the diversity of the bacterial community were thus analyzed.Additionally,the various bacterial functional genes were annotated using COG databases.The results revealed a rich and evenly distributed bacterial species composition in the rhizosphere soil of naked barley,which comprehensively mirrors the bacterial community structure of the samples.The bacterial community of healthy samples was closest to samples with 5%incidence of root rot.Additionally,as the incidence rate of root rot increased,the bacterial communities diverged more significantly from those of healthy samples.The number of bacterial species in diseased rhizosphere soil samples increased initially and then decreased,as the root rot incidence increased.Also,the relative abundance of Proteobacteria initially increased and then decreased with increased root rot,incidence,while a contrasting trend was observed for Chloroflexi,Acidobacteria,Bacteroidetes,Actinobacteria and Gemmatimonadetes.Similarly,the relative abundance of Bacillales,Nitrospirales,and their commonly-present families Gemmatimonadaceae,Bacillaceae,Nitrospiraceae,as well as the genera Gemmatimonas,Bacillus,and Nitrospira,including Bacillus simplex,showed an initial increase followed by a decrease.Conversely,the relative abundance of Acinetobacter calcoaceticus and Stenotrophomonas rhizophila initially decreased and then increased.The abundance of functional genes related to bacterial amino acid transport and metabolism,transcription,lipid transport and metabolism,posttranslational modification,protein turnover,chaperones in the rhizosphere soil of naked barley decreased due to the occurrence of root rot,while the abundance of genes involved in signal transduction mechanisms and energy production and conversion increased.关键词
青稞/根腐病/群落结构/物种多样性/高通量Key words
naked barley/root rot/community structure/species diversity/high-throughput引用本文复制引用
李雪萍,许世洋,李建军,漆永红..青稞根腐病根际土壤细菌多样性及群落结构变化规律[J].草业学报,2025,34(5):118-129,12.基金项目
国家自然科学基金项目(32060037)和甘肃省农业科学院重点研发计划(2023GAAS22)资助. （32060037）
