草业学报2025，Vol.34Issue(5)：130-145,16.DOI:10.11686/cyxb2024253
玉米器官组织内生细菌和真菌群落多样性
Diversity of endophytic bacterial and fungal communities in different maize organs
摘要
Abstract
The relationship between the microenvironment of different tissues of maize and the diversity of endophytic bacteria is of great significance for screening functional microorganisms,such as compatible growth-promoting bacteria or biocontrol bacteria.In this study,high-throughput sequencing technology was used to investigate the diversity of endophytic bacterial and fungal communities in various maize organs and tissues.The results showed that the maize endophytic bacterial community comprised 31 phyla,93 classes,192 orders,340 families,and 404 genera.Proteobacteria and Firmicutes were the dominant bacterial phyla,and Lachnospira,Rhizobium,Enterobacter,and Sphingomonas were the dominant bacterial genera.Some endophytic bacteria were more abundant in some organs than in others,indicating that some bacterial populations had endogenous specificity for particular tissue microenvironments.Comparing the dominant bacterial genera among various organs and tissues,the unique bacterial taxa in the roots were Micrococcus and uncultured_bacterium_f_Muribaculaceae.The unique bacterial taxa in the kernels were Staphylococcus,uncultured_bacterium_f_Muribaculaceae,Delftia,Brevundimonas,Streptococcus,and Clostridium_sensu_stricto_1.The unique bacterial group in the leaves was mainly Pseudomonas,and no unique bacterial genus was detected in the stems.Beta diversity analyses showed that the microbial community composition was similar in the roots,stems,and leaves of maize,but different in the grain.The endophytic fungal community of maize was composed of 12 phyla,37 classes,84 orders,187 families,and 404 genera.Ascomycota,Basidiomycota and Chytridiomycota were the dominant fungal phyla,Mortierella and Fusarium were the dominant fungal genera,and Rozellomycota was the unique dominant fungal group in the roots.Beta diversity analyses showed that there was little difference in fungal community composition among stems,leaves,and grain of maize,but it was significantly different in the root.In summary,the distribution of endophytic bacteria differs among maize organs and tissues.Compared with the endophytic fungal community,the endophytic bacterial community showed wider variations among the different organs and tissues.关键词
玉米/组织器官/内生细菌/内生真菌/多样性Key words
maize/tissue and organs/endophytic bacteria/endophytic fungi/diversity引用本文复制引用
董晓慧,师尚礼,尹国丽,陈三冬,巩海强,刘林波..玉米器官组织内生细菌和真菌群落多样性[J].草业学报,2025,34(5):130-145,16.基金项目
2024年度农业类省级技术创新引导计划-东西部科技协作专项全株青贮玉米"种+贮+喂"一体化技术集成及推广应用资助. （）
