草业学报2025，Vol.34Issue(5)：159-170,12.DOI:10.11686/cyxb2024226
复合乳酸菌添加剂对低温环境下意大利黑麦草青贮发酵品质的影响
Improvement in the fermentation quality of Italian ryegrass silage by ensiling with combined lactic acid bacteria inoculants at low temperature
摘要
Abstract
Temperature is an important factor affecting ensilage.In cold regions,low temperature can be an adverse environmental condition for ensiling.However,few studies have focused on improving the ensiling process at low temperature to enhance silage quality.The aims of this study were:1)To characterize the lactic acid bacteria(LAB)strains Pediococcus acidilactici LOG9,Pediococcus pentosaceus LO7,Lentilactobacillus coryniformis subsp.LM8,and Lactiplantibacillus plantarum M1,which were isolated from the Tibetan Plateau.2)Determine the effect of adding combinations of these strains on the quality of Italian ryegrass(Lolium multiflorum)silage produced at three temperatures(10,15,25℃).The isolated strains were subjected to morphological,physiological,and biochemical analyses.Four combined inoculants(LO7+LM8,LO7+M1,LOG9+LM8,and LOG9+M1)were added to Italian ryegrass(at 1×105 cfu·g-1 fresh weight).These mixtures were then ensiled for 60 days in laboratory silos(1 L)at different ambient temperatures(10,15,25℃).All the isolates were able to grow normally at 5-20℃,pH 3.5-7.0,with NaCl concentrations of 3.0%and 6.5%w/w.Compared with the three corresponding controls,all the combined LAB inoculants improved the quality of Italian ryegrass silage,as indicated by markedly higher(P<0.05)lactic acid(LA)contents,higher lactic acid/acetic acid(LA/AA),lower pH and ammonia nitrogen(NH3-N)contents,and lower counts of undesirable microorganisms.With ensilage at 10 and 15℃,LOG9+LM8/M1 inoculants performed better than the LO7+LM8/M1 inoculants,as demonstrated by the distinctly higher(P<0.05)LA contents and LA/AA,and lower NH3-N contents.Compared with the silage produced with LOG9+M1,that produced with LOG9+LM8 had significantly(P<0.05)higher LA contents and LAB counts,and lower NH3-N contents.Therefore,the combined inoculant LOG9+LM8 is recommended as the starter culture for producing Italian ryegrass silage at low temperature.关键词
发酵品质/复合乳酸菌添加剂/意大利黑麦草/青贮饲料/低温Key words
fermentation quality/combined lactic acid bacteria inoculant/Italian ryegrass/silage/low temperature引用本文复制引用
王思然,刘蓓一,田吉鹏,程云辉,许能祥,张文洁,王欣,丁成龙..复合乳酸菌添加剂对低温环境下意大利黑麦草青贮发酵品质的影响[J].草业学报,2025,34(5):159-170,12.基金项目
国家自然科学基金青年科学基金项目(32301500),江苏现代农业产业技术体系建设专项资金(JATS[2023]398),农业农村部种养结合重点实验室开放课题基金(202303)和江苏省大型科学仪器开放共享自主研究课题资助. （32301500）
