大学化学2025，Vol.40Issue(4)：390-398,9.DOI:10.12461/PKU.DXHX202405147
荧光定量分析实验的改进
Improvement of Fluorescence Quantitative Analysis Experiment:Simultaneous Determination of Rhodamine 6G and Rhodamine 123 in Food Using Chemometrics-Assisted Three-Dimensional Fluorescence Method
摘要
Abstract
The existing instrument analysis experiment,"Molecular fluorescence method for the determination of rhodamine B",is overly simplistic and dose not account for real-world complexities.To address these issues,this improved experiment utilizes three-dimensional fluorescence scanning to acquire sample data.Instead of complex preprocessing steps,chemometric algorithms are applied to extract the pure signals of the target analytes,enabling the simultaneous determination of rhodamine 6G and rhodamine 123 in dyed chili.This improvement enhances students'ability to approach problems comprehensively and develop innovative solutions.关键词
罗丹明6G/罗丹明123/三维荧光/化学计量学/染色辣椒Key words
Rhodamine 6G/Rhodamine 123/Three-dimensional fluorescence method/Chemometrics/Dyed chili分类
社会科学引用本文复制引用
文密,贾宝硕,柴永琪,王童,刘剑波,吴海龙..荧光定量分析实验的改进[J].大学化学,2025,40(4):390-398,9.基金项目
2024年湖南省普通本科高校教学改革研究项目(202401000432) （202401000432）
国家自然科学基金(22204049) （22204049）
湖南省教育厅科学研究重点项目(23A0028) （23A0028）
