南方医科大学学报2025，Vol.45Issue(5)：911-920,10.DOI:10.12122/j.issn.1673-4254.2025.05.03
电针预处理通过调节肠道-大脑轴及Nrf2/HO-1信号通路抑制铁死亡减轻大鼠脑缺血再灌注损伤
Electroacupuncture pretreatment alleviates cerebral ischemia-reperfusion injury in rats by inhibiting ferroptosis through the gut-brain axis and the Nrf2/HO-1 signaling pathway
摘要
Abstract
Objective To investigate the neuroprotective effects of electroacupuncture(EA)preconditioning against cerebral ischemia-reperfusion injury(CIRI)mediated by gut microbiota modulation,Nrf2/HO-1 pathway activation,and ferroptosis suppression.Methods Adult male SD rats were divided into sham operation group,CIRI model group,and EA preconditioning group.In the latter two groups,rat models of CIRI were established by middle cerebral artery occlusion(MCAO),and in EA preconditioning group,EA was applied at Baihui(DU20)and Zusanli(ST36)for 3 days before modeling.Neurological deficits,cerebral infarction,and hippocampal pathology of the rats were evaluated using behavioral tests,TTC staining,and Nissl and HE staining,and the oxidative stress markers(MDA,ROS,and SOD),apoptosis/ferroptosis-related proteins(Bax,Bcl-2,GPX4,and SLC7A11),and changes in gut microbiota were analyzed.Results EA preconditioning significantly reduced neurological deficits,decreased infarct volume,promoted hippocampal neuronal survival,and improved structural integrity of the hippocampal neurons in MCAO rats.EA preconditioning also significantly lowered MDA and ROS and increased SOD levels,upregulated Bcl-2,GPX4,and SLC7A11 expressions,and downregulated Bax expression in the hippocampal tissue of the rats,causing also activation of Nrf2/HO-1 signaling and improvement of gut microbiota composition.Conclusion EA preconditioning alleviates CIRI in rats by suppressing ferroptosis and apoptosis,enhancing antioxidant defenses via activating Nrf2/HO-1 signaling,and regulating the gut-brain axis.关键词
电针/脑缺血再灌注损伤/氧化应激/肠道微生物/铁死亡Key words
electroacupuncture/cerebral ischemia-reperfusion injury/oxidative stress/gut microbiota/ferroptosis引用本文复制引用
张安邦,孙秀颀,庞博,吴远华,时靖宇,张宁,叶涛..电针预处理通过调节肠道-大脑轴及Nrf2/HO-1信号通路抑制铁死亡减轻大鼠脑缺血再灌注损伤[J].南方医科大学学报,2025,45(5):911-920,10.基金项目
国家自然科学基金(82060896) （82060896）
贵州省基础研究项目(QianKeHeJiChu-ZK[2021]General 505) （QianKeHeJiChu-ZK[2021]General 505）
贵州省教育厅青年科技人才成长项目(QianJiaoHe KY Zi[2021]210) （QianJiaoHe KY Zi[2021]210）
贵州省卫健委科技基金项目(gzwkj2021-072) （gzwkj2021-072）
贵州省中医药管理局中药与民族医药科学技术研究项目(QZYY-2021-015) （QZYY-2021-015）
贵州中医药大学第一附属医院博士启动基金[GYZYYFY-BS-2021(01),GYZYYFY-BS-2021(02)]Supported by National Natural Science Foundation of China(82060896). （01）
