南方医科大学学报2025，Vol.45Issue(5)：921-928,8.DOI:10.12122/j.issn.1673-4254.2025.05.04
眼针疗法通过上调METTL3介导的m6A甲基化修饰促进脑皮层血管新生进而改善脑缺血再灌注损伤大鼠的神经功能
Eye acupuncture improves neural function in rats with cerebral ischemia-reperfusion injury by promoting angiogenesis via upregulating METTL3-mediated m6 A methylation
摘要
Abstract
Objective To evaluate the effect of eye acupuncture on neural function and angiogenesis of ischemic cerebral tissue in rats,and explore the roles of METTL3-mediated m6A methylation and the HIF-1α/VEGF-A signal axis in mediating this effect.Methods Fifty SD rats were randomized into normal control group,sham-operated group,model group,eye acupuncture group and DMOG(a HIF-1α agonist)group.Rat models of cerebral ischemia/reperfusion injury(CIRI)were established using a modified thread thrombus method,and the changes in neurological deficits of the rats after interventions were evaluated.TTC and Nissl staining were used to examine the changes in infarction size and neuronal injury,and cerebral angiogenesis was detected by double-immunofluorescence staining.m6A methylation modification level in the brain tissue was detected by ELISA,and RT-qPCR and Western blotting were used to detect the mRNA and protein expressions of METTL3 and HIF-1α/VEGF-A.Results Compared with the control and sham-operated rats,the CIRI rats had significantly higher neurological deficit scores with larger cerebral infarction area,a greater number of CD31-and EDU-positive new vessels,higher expression levels of HIF-1α and VEGF-A,reduced number of Nissl bodies and m6A methylation level,and lowered METTL3 protein and mRNA expressions.All these changes were significantly improved by interventions with eye acupuncture after modeling or intraperitoneal injections of DMOG for 7 consecutive days prior to modeling,and the effects of the two interventions were similar.Conclusion Eye acupuncture can improve neurological deficits in CIRI rat models possibly by promoting cortical angiogenesis via upregulating METTL3-mediated m6A methylation and regulating the HIF-1α/VEGF-A signal axis.关键词
眼针/脑缺血再灌注损伤/m6A甲基化修饰/HIF-1α/VEGF-A信号轴/血管新生Key words
eye acupuncture/cerebral ischemia reperfusion injury/m6A methylation modification/HIF-1α/VEGF-A signaling axis/angiogenesis引用本文复制引用
浦延鹏,王震,储浩然..眼针疗法通过上调METTL3介导的m6A甲基化修饰促进脑皮层血管新生进而改善脑缺血再灌注损伤大鼠的神经功能[J].南方医科大学学报,2025,45(5):921-928,8.基金项目
国家自然科学基金(81774399) （81774399）
储浩然全国名老中医药专家传承工作室项目(国中医药人教函[2022]75号) （国中医药人教函[2022]75号）
安省中医药领军人才项目(中医药发展秘[2018]23号) （中医药发展秘[2018]23号）
安徽省自然科学基金青年项目(2308085QH293) （2308085QH293）
安徽省教育厅重点项目(2023AH050829) （2023AH050829）
安徽省中医药传承创新科研项目(2024CCCX118) （2024CCCX118）
林咸明安徽省长三角名医工作室(皖中医药发展秘[2023]16号) （皖中医药发展秘[2023]16号）
安徽中医药大学国家中医药管理局中医基础理论高水平重点学科开放课题(ZYJCLLYB-03)Supported by National Natural Science Foundation of China(81774399). （ZYJCLLYB-03）
