南方医科大学学报2025，Vol.45Issue(5)：954-961,8.DOI:10.12122/j.issn.1673-4254.2025.05.07
绒毛样蛋白VILL通过与LMO7蛋白相互作用抑制鼻咽癌细胞的增殖
Villin-like protein VILL suppresses proliferation of nasopharyngeal carcinoma cells by interacting with LMO7 protein
摘要
Abstract
Objective To elucidate the molecular mechanism by which villin-like protein VILL(VILL)inhibits proliferation of nasopharyngeal carcinoma(NPC)cells.Methods Co-immunoprecipitation(CO-IP)assay,mass spectrometry,Western blotting,immunofluorescence staining,and GST pull-down assay were employed to identify and confirm the protein interacting with VILL that had the highest abundance in NPC cell lines.Transgenic experiments were conducted in both NPC cell lines and nude mice to validate the regulatory role of VILL and its target protein in NPC proliferation.Immunohistochemistry was utilized to assess the correlation of the expression levels of VILL and its target protein in clinical tissue specimens of NPC with the clinical features of the patients.Results In NPC cell lines(HONE1 EBV and S18),VILL was found to interact most abundantly with the E3 ubiquitin ligase LMO7,and both proteins co-localized in the cytoplasm with direct interactions.Overexpression of LMO7 partially counteracted the inhibitory effect of VILL on NPC cell proliferation.The expression of VILL was significantly downregulated in 136 NPC tissue samples compared to 67 non-cancerous nasopharyngeal tissues(P<0.00001)with close correlation with clinical T stage(P=0.04),N stage(P=0.01),and M stage(P=0.013),whereas LMO7 was highly expressed in all the NPC tissues.Conclusion VILL overexpression inhibits NPC proliferation probably by suppressing the oncogenic function of LMO7.关键词
鼻咽癌/VILL蛋白/LMO7蛋白/蛋白相互作用/增殖Key words
nasopharyngeal carcinoma/villin-like protein VILL/LMO7 protein/protein interaction/cell proliferation引用本文复制引用
曾玉梅,李继科,黄仲曦,周毅波..绒毛样蛋白VILL通过与LMO7蛋白相互作用抑制鼻咽癌细胞的增殖[J].南方医科大学学报,2025,45(5):954-961,8.基金项目
湖南省自然科学基金(2023JJ30068) （2023JJ30068）
长沙市自然科学基金(kq2208490) （kq2208490）
中山市社会公益与基础研究项目(K2021-0043) （K2021-0043）
