南方医科大学学报2025，Vol.45Issue(5)：962-968,7.DOI:10.12122/j.issn.1673-4254.2025.05.08
丰富环境通过调控前扣带回皮层锥体神经元兴奋性缓解束缚应激诱导的小鼠焦虑样行为
Enriched environment reduces pyramidal neuron excitability in the anterior cingulate cortex to alleviate restraint stress-induced anxiety-like behaviors in mice
摘要
Abstract
Objective To investigate the mechanism by which the pyramidal neurons of the anterior cingulate cortex(ACC)modulate the effects of enriched environment(EE)for relieving anxiety-like behaviors in mice.Methods C57BL/6J mice were randomly divided into control group,restraint stress(RS)group,and RS+EE group(n=8).The mice in the latter two groups were subjected to RS for 2 h daily for 3 days,and those in RS+EE group were housed in an EE during modeling.Anxiety-like behaviors of the mice were evaluated using the elevated plus-maze tests(EPM)and open field test(OFT).Changes in c-Fos expression in the ACC of the mice were detected with immunofluorescence assay,and pyramidal neuron excitability in the ACC(PynACC)was measured using patch-clamp technique.The miniature excitatory and inhibitory postsynaptic currents(mEPSC and mIPSC,respectively)were analyzed to assess synaptic transmission changes.Results Behavioral tests showed obvious anxiety-like behaviors in RS mice,and such behavioral changes were significantly improved in RS+EE mice.Immunofluorescence staining revealed significantly increased c-Fos expression in the ACC in RS mice but lowered c-Fos expression in RS+EE group.Compared with the control mice,the RS mice showed increased action potential firing rate of PynACC,which was significantly reduced in RS+EE group.Compared with the RS mice,the RS+EE mice showed also decreased frequency of mEPSCs of PynACC,but the amplitude exhibited no significant changes.No obvious changes in the frequency or amplitude of mIPSCs were observed in RS+EE mice.Conclusion EE reduces excitability of PynACC to alleviate anxiety-like behaviors induced by RS in mice.关键词
丰富环境/前扣带回皮层/锥体神经元/焦虑/兴奋性Key words
enriched environment/anterior cingulate cortex/pyramidal neuron/anxiety/excitability引用本文复制引用
陈昌锋,方勤,高银环,王烈成,陈磊..丰富环境通过调控前扣带回皮层锥体神经元兴奋性缓解束缚应激诱导的小鼠焦虑样行为[J].南方医科大学学报,2025,45(5):962-968,7.基金项目
安徽省自然科学基金(2408085MH189) （2408085MH189）
安徽省高等学校科学研究项目(2024AH051017,KJ2021A0202) （2024AH051017,KJ2021A0202）
安徽省高校中青年教师培养行动项目(YQYB2024031) （YQYB2024031）
安徽中医药大学第一附属医院临床科学研究项目(2021yfylc14) （2021yfylc14）
安徽省生物医学基础与应用科技公共服务平台2023年大型仪器设备开放基金(2023-04) （2023-04）
安徽省级大学生创新创业训练计划项目(S202410366078) （S202410366078）
安徽医科大学科研平台基地升级计划(2021xkjT048) （2021xkjT048）
