南方医科大学学报2025，Vol.45Issue(5)：986-994,9.DOI:10.12122/j.issn.1673-4254.2025.05.11
活血疏风颗粒通过抑制TLR4/NF-κB通路改善慢性偏头痛小鼠的中枢敏化
Huoxue Shufeng Granule alleviates central sensitization in mouse models of chronic migraine by inhibiting the TLR4/NF-κB signaling pathway
摘要
Abstract
Objective To investigate the therapeutic mechanism of Huoxue Shufeng Granules(HXSFG)for alleviating central sensitization in a mouse model of chronic migraine(CM).Methods We analyzed the main chemical components of HXSFG through literature review and explored their pharmacological mechanisms by bioinformatics analyses.In a male C57BL/6J mouse model of CM established by intraperitoneal injections of nitroglycerin(10 mg/kg)every other day(5 injections),the effects of gavage with low,and high doses of HXSFG or intraperitoneal injections of topiramate for ameliorating central sensitization were evaluated using Von Frey test and a hot plate apparatus;the changes in expressions of inflammatory factors,the proteins in the TLR4/NF-κB signaling pathway,and activation of c-Fos and CGRP were detected using RT-qPCR,Western blotting and immunofluorescence staining.Results Network pharmacology analysis suggested that the main active components in HXSFG for alleviating CM included formononetin,paeoniflorin,quercetin,and tanshinone.Gene Ontology(GO)enrichment analysis identified 492 GO entries,comprising 366 biological processes,46 cellular components,and 80 molecular functions.KEGG pathway enrichment analysis indicated that the Toll-like receptor and NF-κB signaling pathways were crucial in mediating the therapeutic effects of HXSFG on CM.In the mouse models of CM,both topiramate and HXSFG treatments alleviated the symptoms of central sensitization,evidenced by improved mechanical and thermal pain thresholds in the mice.HXSFG significantly reduced the expression of c-Fos and CGRP,improved inflammatory markers,and downregulated the expressions of TLR4,p-NF-κB,IL-1β,and TNF-α proteins in the mouse models.Conclusion HXSFG effectively alleviates central sensitization in CM mice by modulating the inflammatory pathways and inhibiting the TLR4/NF-κB signaling pathway,suggesting its potential as a therapeutic option for CM.关键词
活血疏风颗粒/慢性偏头痛/TLR4/NF-κB/网络药理学Key words
Huoxue Shufeng Granules/chronic migraine/TLR4/NF-κB/network pharmacology引用本文复制引用
梁晓涛,熊一凡,刘雪琪,梁小珊,朱晓煜,谢炜..活血疏风颗粒通过抑制TLR4/NF-κB通路改善慢性偏头痛小鼠的中枢敏化[J].南方医科大学学报,2025,45(5):986-994,9.基金项目
第二届全国名中医工作室(G724290126) （G724290126）
中西医结合情志病防治重大成果培育(G624290511) （G624290511）
2023年度省基础与应用基础研究基金企业联合基金(公共卫生与医药健康领域)(2023A1515220030) （公共卫生与医药健康领域）
重大疑难疾病中西医临床协作项目 （）
