南方医科大学学报2025，Vol.45Issue(5)：1003-1012,10.DOI:10.12122/j.issn.1673-4254.2025.05.13
银翘散配方颗粒与传统汤剂在抗炎、抗菌及镇痛药效的差异比较
Comparison of anti-inflammatory,antibacterial and analgesic activities of formulated granules versus traditional decoction of Yinqiao Powder
摘要
Abstract
Objective To compare the anti-inflammatory,antibacterial and analgesic effects of Yinqiao Powder(YQS)formulated granules and decoction.Methods We first evaluated the anti-inflammatory effects of the two dosage forms of YQS in a LPS-induced RAW 264.7 cell model using RT-qPCR and Western blotting.We further constructed zebrafish models of inflammation by copper sulfate exposure,caudal fin transection,or LPS and Poly(I:C)microinjection,and evaluated anti-inflammatory effects of YQS granules and decoction by examining neutrophil aggregation and HE staining findings.In a mouse model of acute lung injury(ALI)induced by intratracheal LPS instillation,the effects of YQS gavage at 10,15,and 20 g/kg on lung pathologies were evaluated by calculating lung wet-dry weight ratio and using HE staining,ELISA and Western blotting.The microbroth dilution method was used to evaluate the antibacterial effect of YQS.Mouse pain models established by hot plate and intraperitoneal injection of glacial acetic acid were used to evaluate the analgesic effects of YQS at 10,15,and 20 g/kg.Results Both YQS granules and decoction significantly reduced TNF-α,IL-6,and IL-1β expressions and p-STAT3(Tyr 705)phosphorylation level in LPS-induced RAW 264.7 cells,and obviously inhibited neutrophil aggregation in the zebrafish models.In ALI mice,YQS granules and decoction effectively ameliorated lung injury,lowered lung wet-dry weight ratio,and reduced p-STAT3(Tyr 705)expression and TNF-α and IL-6 levels.YQS produced obvious antibacterial effect at the doses of 15.63 and 31.25 mg/mL,and significantly reduced body torsion and increased pain threshold in the mouse pain models.Conclusion The two dosage forms of TQS have similar anti-inflammatory,antibacterial and analgesic effects with only differences in their inhibitory effect on TNF-α,IL-6 and IL-1β mRNA expressions in LPS-induced RAW 264.7 cells.关键词
银翘散/中药配方颗粒/药物等效性Key words
Yinqiao Powder/formulated granules of traditional Chinese medicine/pharmacological equivalence引用本文复制引用
郭卓琳,刘俊珊,张智恒,郭新邓,杨苇巍,梁芷晴,区锦莹,曹惠慧,卢子滨,余林中..银翘散配方颗粒与传统汤剂在抗炎、抗菌及镇痛药效的差异比较[J].南方医科大学学报,2025,45(5):1003-1012,10.基金项目
广东省基础与应用基础研究基金(2022A1515220081) （2022A1515220081）
