南方医科大学学报2025，Vol.45Issue(5)：1023-1030,8.DOI:10.12122/j.issn.1673-4254.2025.05.15
Orexin-A通过调节促离子型谷氨酸受体促进脊髓损伤大鼠运动功能恢复
Orexin-A promotes motor function recovery of rats with spinal cord injury by regulating ionotropic glutamate receptors
摘要
Abstract
Objective To investigate the effect of orexin-A-mediated regulation of ionotropic glutamate receptors for promoting motor function recovery in rats with spinal cord injury(SCI).Methods Thirty-six newborn SD rats(aged 7-14 days)were randomized into 6 groups(n=6),including a normal control group,a sham-operated group,and 4 SCI groups with daily intrathecal injection of saline,DNQX,orexin-A,or orexin-A+DNQX for 3 consecutive days after PCI.Motor function of the rats were evaluated using blood-brain barrier(BBB)score and inclined plane test 1 day before and at 1,3,and 7 days after SCI.For patch-clamp experiment,spinal cord slices from newborn rats in the control,sham-operated,SCI,and SCI+orexin groups were prepared,and ventral horn neurons were acutely isolated to determine the reversal potential and dynamic indicators of glutamate receptor-mediated currents under glutamate perfusion.Results At 3 and 7 days after SCI,the orexin-A-treated rats showed significantly higher BBB scores and grip tilt angles than those with other interventions.Compared with those treated with DNQX alone,the rats receiving the combined treatment with orexin and DNQX had significantly higher BBB scores and grip tilt angles on day 7 after PCI.In the patch-clamp experiment,the ventral horn neurons from SCI rat models exhibited obviously higher reversal potential and greater rise slope of glutamate current with shorter decay time than those from sham-operated and orexin-treated rats.Conclusion Orexin-A promotes motor function recovery in rats after SCI possibly by improving the function of the ionotropic glutamate receptors.关键词
Orexin-A/促离子型谷氨酸受体/脊髓损伤/脊髓腹角神经元/膜片钳记录Key words
orexin-A/ionotropic glutamate receptor/spinal cord injury/spinal cord ventral horn neurons/patch-clamp recording引用本文复制引用
何光侣,郑超,储婉玉,李妍,盛鑫,罗浩,徐爱萍,卞命杰,张环环,汪萌芽..Orexin-A通过调节促离子型谷氨酸受体促进脊髓损伤大鼠运动功能恢复[J].南方医科大学学报,2025,45(5):1023-1030,8.基金项目
国家自然科学基金(31200828) （31200828）
安徽省自然科学基金(2108085MC91) （2108085MC91）
安徽省高校学科拔尖人才学术资助项目(gxbjZD2021061) （gxbjZD2021061）
安徽省教育厅自然科学研究项目(2024AH051954,2024yjsmsgzs043,2022msgzs037,2022AH051245,2023AH051778)Supported by National Natural Science Foundation of China(31200828). （2024AH051954,2024yjsmsgzs043,2022msgzs037,2022AH051245,2023AH051778）
