广东农业科学2025，Vol.52Issue(3)：1-13,13.DOI:10.16768/j.issn.1004-874X.2025.03.001
解淀粉芽孢杆菌脂肽提取物对番茄链格孢菌的抑菌机制研究
Study on Antifungal Mechanism of Lipopeptide Extract from Bacillus amyloliquefaciens Against Alternaria alternata in Tomato
摘要
Abstract
[Objective]This study elucidated the antifungal rate and mechanism of lipopeptide extracts from Bacillus amyloliquefaciens against Alternaria alternata in tomatoes,aiming to provide scientific basis for green control of black spot disease in tomatoes.[Method]A alternata,the dominant pathogen of tomato black spot,was taken as the research object.The antifungal mechanism of B.amyloliquefaciens lipopeptide extract on A.alternata was investigated through in-dish antifungal test,scanning electron microscope(SEM)observation test,in vitro fruit test and transcriptomic analysis.[Result]In-dish antifungal test showed that the inhibition rate of B.amyloliquefaciens lipopeptide extract on the growth of A.alternata colony was 56.00%and the inhibition rate on mycelium biomass was 60.14%on the seventh day of culture.SEM showed that lipopeptide extracts treatment could lead to distortion and collapse of A.alternata mycelium and spores.In vitro fruit test showed that the diameter of tomato black spot disease lesion decreased by 89.97%after inoculation with lipopeptide extract.Compared with the control group,the incidence of black spot decreased from 17.94%to 6.25%,and the control effect of black spot disease was 65.16%.Transcriptome sequencing was performed on A.alternata mycelium treated with lipopeptide extract and untreated,and the differential genes of MAPK signaling pathway and glycolysis/gluconeogenesis signaling pathway were analyzed,respectively.And it was found that the gene encoding Ras homologous gene family A(Rho1)was significantly up-regulated.The genes encoding protein Ste50,glucose-6-phosphatase,hexokinase,pyruvate carboxylase and 6-phosphofructokinase were significantly down-regulated.These up-regulated and down-regulated genes may be the key genes related to the inhibition of A.alternata growth by lipopeptide extract.[Conclusion]The lipopeptide extract can inhibit the growth of A.alternata by destroying spore and mycelium structure and regulating the expression of key genes.关键词
脂肽提取物/链格孢菌/番茄/转录组/关键基因/抑菌机制/黑斑病Key words
lipopeptide extract/Alternaria alternata/tomato/transcriptome/key genes/antifungal mechanism/black spot disease分类
农业科技引用本文复制引用
范盈盈,戴莹莹,张媛媛,沈婷婷,王艳,王沛成,刘峰娟,王成..解淀粉芽孢杆菌脂肽提取物对番茄链格孢菌的抑菌机制研究[J].广东农业科学,2025,52(3):1-13,13.基金项目
国家自然科学基金(32260634) （32260634）
新疆蔬菜产业技术体系专项(XJARS-07) （XJARS-07）
新疆农业科学院稳定支持专项(xjnkywdzc-2023003-1) （xjnkywdzc-2023003-1）
