广东农业科学2025，Vol.52Issue(3)：14-24,11.DOI:10.16768/j.issn.1004-874X.2025.03.002
花生PLATZ基因家族鉴定及其在盐胁迫和干旱胁迫下的表达分析
Identification of the PLATZ Gene Family in Peanut and Its Expression Analysis under Salt Stress and Drought Stress
摘要
Abstract
[Objective]Plant AT-rich sequence and zinc-binding(PLATZ)transcription factors are plant-specific,zinc-dependent DNA-binding proteins that play a role in regulating plant growth and environmental stress responses.To clarify the characteristics of the PLATZ family members in peanut(Arachis hypogaea),this study screened and analyzed the expression of the identified PLATZ gene family members in peanut.[Method]Using peanut genome data,the PLATZ gene family members in peanut were identified,and bioinformatics methods were used to systematically analyze the physicochemical properties,phylogenetic trees,protein conservative motifs,cis-acting elements,and protein interactions of this gene family members.And the expression patterns of peanut PLATZ genes under salt and drought stress were analyzed using RT-qPCR.[Result]A total of 13 AhPLATZ genes were identified in peanuts,which are unevenly distributed across 7 chromosomes.The AhPLATZ proteins range from 194 to 260 amino acids in length,with molecular weights between 22.00 and 29.49 kD,instability indices between 43.42 and 73.46,aliphatic indices between 47.46 and 83.92,and protein hydrophilicity between-1.094 and-0.345,indicating that they are all hydrophilic proteins.The AhPLATZ proteins are primarily localized in the nucleus and chloroplasts.Based on phylogenetic relationships,the AhPLATZ genes were categorized into 4 groups,with genes within the same group typically having similar motif compositions and numbers of exons or introns.The collinearity results suggest that the expansion of AhPLATZ gene members was achieved through gene duplication after diversification.Additionally,the promoter regions of AhPLATZ include various cis-acting elements associated with stress response and hormone regulation.The results of the RT-qPCR analysis indicate that AhPLATZ4,AhPLATZ5,and AhPLATZ11 exhibit significantly induced expression under salt stress,while AhPLATZ4 and AhPLATZ5 actively respond to drought stress.[Conclusion]Thirteen members of the PLATZ gene family have been identified in peanuts,among which AhPLATZ4 and AhPLATZ5 actively respond to both salt stress and drought stress.These findings provide a basis for further functional investigations of the AhPLATZ genes in peanuts.关键词
花生/PLATZ/基因家族/盐胁迫/干旱胁迫/表达分析Key words
peanut/PLATZ/gene family/salt stress/drought stress/expression analysis分类
农业科技引用本文复制引用
陈新起,冯恩友,林雄健,胡梦琴,唐滨泉,吴小丽,谭家壮,陈少菜,宋昌艺,李嘉兴..花生PLATZ基因家族鉴定及其在盐胁迫和干旱胁迫下的表达分析[J].广东农业科学,2025,52(3):14-24,11.基金项目
国家现代农业产业技术体系专项(CARS-13) （CARS-13）
广东省现代农业产业技术体系创新团队建设项目(油料作物产业技术体系)(2024CXTD06) （油料作物产业技术体系）
