广东医学2025，Vol.46Issue(4)：502-507,6.DOI:10.13820/j.cnki.gdyx.20243258
大叶茜草素对宫颈癌细胞增殖、黏附及糖酵解水平的调控
Molecular mechanisms underlying the inhibitory effects of mollugin on cervical cancer cell proliferation,adhe-sion,and glycolysis via the PI3K/AKT pathway
摘要
Abstract
Objective To investigate the regulatory effects of mollugin on the proliferation,adhesion,glycolytic activity,and PI3K/AKT signaling pathway in human cervical cancer cells.Methods Human cervical cancer HeLa cells were cultured in vitro and treated with various concentrations of mollugin(0,12.5,25,50,and 100 μmol/L).The cell viability was assessed using the CCK-8 assay to determine the optimal concentration,which was found to be 50 μmol/L.HeLa cells were then divided into five groups,Control Group(0 μmol/L mollugin),Experimental Group(50 μmol/L mollugin),Positive Drug Group(20 μg/mL 5-fluorouracil),Activator Group(50 μmol/L mollugin+10 μmol/L PI3K/AKT pathway activator SC-79)and Inhibitor Group(50 μmol/L mollugin+2 μmol/L PI3K/AKT pathway inhib-itor LY294002).All treatments were applied for 24 hours.Cell proliferation was measured using the EdU assay;cell ad-hesion was evaluated via adhesion experiments;lactate and glucose levels were determined using specific assay kits;and the expression levels of key proteins-including hexokinase 2(HK2),lactate dehydrogenase A(LDHA),glyceraldehyde-3-phosphate dehydrogenase(GAPDH),phosphorylated PI3 K(p-PI3 K),and phosphorylated AKT(p-AKT)-were analyzed by Western blotting.Results The CCK-8 assay confirmed that 50 μmol/L was the optimal concentration of mollugin for intervention.Compared to the control group,both the experimental and positive drug groups exhibited signifi-cant reductions in cell proliferation,adhesion,lactate and glucose levels,and protein expression levels of HK2,LDHA,GAPDH,p-PI3K,and p-AKT(P＜0.05).Furthermore,the inhibitor group showed a further significant decrease in these indices compared to the experimental group(P＜0.05),whereas the activator group exhibited a significant increase in these parameters relative to the experimental group(P＜0.05).Conclusion Mollugin exerts an inhibitory effect on HeLa cell proliferation,adhesion,and glycolytic activity by suppressing the PI3K/AKT signaling pathway,suggesting its potential as a therapeutic agent for cervical cancer.关键词
宫颈癌/大叶茜草素/增殖/黏附/糖酵解/磷脂酰肌醇-3激酶/丝氨酸苏氨酸蛋白激酶信号通路Key words
cervical cancer/mollugin/proliferation/adhesion/glycolysis/PI3K/AKT signal pathways分类
基础医学引用本文复制引用
赵博,王应,王献红..大叶茜草素对宫颈癌细胞增殖、黏附及糖酵解水平的调控[J].广东医学,2025,46(4):502-507,6.基金项目
河南省中医药科学研究专项课题计划(2023ZY3099) （2023ZY3099）
