干旱地区农业研究2025，Vol.43Issue(3)：52-59,8.DOI:10.7606/j.issn.1000-7601.2025.03.06
过表达黄瓜CsAGO1c基因降低拟南芥植株的耐旱性
Overexpression of the cucumber CsAGO1c gene reduces drought tolerance in Arabidopsis thaliana
摘要
Abstract
CsAGO1c was cloned from the inbred line of cucumis variety'Xintaimici',and its expression was analyzed under drought stress.An overexpression vector for CsAGO1c was constructed and used to transform Arabi-dopsis via Agrobacterium-mediated transformation.The phenotypic and physiological changes of the CsAGO1c-over-expressing plants were then studied under drought stress conditions.The results showed that the expression level of CsAGO1c in cucumber root first decreased and then increased.The expression level of CsAGO1c was down-regulated by 57%at 6 h,down-regulated by 64%at 12 h,and up-regulated by 4.24 times at 24 h.The expression level of CsAGO1c in the leaves decreased first,then increased and then decreased,and the expression level of CsAGO1c in-creased 1.16 times at 18 h.Overexpressed Arabidopsis seeds germinated earlier than the wild type.The penetration test showed that 400 mmol·L-1 mannitol treatment significantly inhibited the growth of OE2 root length,which was 54%that of WT.The water loss rate of isolated leaves was 1.21,1.27,and 1.25 times higher than that of the wild type(WT)at 3,4,and 5 hours,respectively,showing a significantly greater loss compared to WT at all three time points.Additionally,under drought stress,the activities of superoxide dismutase and peroxidase in the overex-pressing Arabidopsis lines were approximately 64%and 51%of those in WT,respectively,both significantly lower than WT levels.Meanwhile,the malondialdehyde content in OE2 was about three times higher than that of WT,in-dicating a marked increase.These findings suggest that heterologous expression of CsAGO1c reduces drought toler-ance in Arabidopsis.This study provides valuable theoretical insight into the function of the CsAGO1c gene in cu-cumber and offers a foundation for breeding new drought-resistant cucumber varieties.关键词
黄瓜/CsAGO1c基因/过表达/拟南芥/耐旱性Key words
cucumber/CsAGO1c gene/overexpression/Arabidopsis/drought tolerance分类
农业科技引用本文复制引用
管丽丽,况勇,肖逸,孙照龙,甘德芳..过表达黄瓜CsAGO1c基因降低拟南芥植株的耐旱性[J].干旱地区农业研究,2025,43(3):52-59,8.基金项目
安徽省自然科学研究重点项目(2023AH051056) （2023AH051056）
