国际医药卫生导报2025，Vol.31Issue(10)：1599-1604,6.DOI:10.3760/cma.j.cn441417-20240722-10003
子痫前期患者胎盘组织中circDLG1与HIF-1α、PLGF、MVD的表达及关系
Expressions of circDLG1,HIF-1α,PLGF,and MVD in placental tissue of patients with pre-eclampsia and their relationship
摘要
Abstract
Objective To investigate the expressions of circular RNA DLG1(circDLG1),hypoxia-inducible factor-1α(HIF-1α),placental growth factor(PLGF),and placental microvascular density(MVD)in placental tissue of patients with pre-eclampsia(PE)and their relationship.Methods A total of 116 pregnant women with PE who delivered in Department of Obstetrics,The Second Affiliated Hospital of Xi'an Medical University from January 2020 to December 2023 were selected as an observation group;they were(29.15±5.82)years old;there were 72 primiparas,and 44 multiparas;their gestational age was(37.02±2.14)weeks.A total of 116 healthy pregnant women in the same period were selected as a control group;they were(28.72±5.36)years old;there were 78 primiparas and 38 multiparas;their gestational age was(36.68±2.02)weeks.The clinical data,including age,BMI before pregnancy and at delivery,parity,gestational week,diastolic and systolic blood pressures,blood urea nitrogen,lactate dehydrogenase(LDH),creatinine(Cr),urea protein,platelet count(PLT),mean platelet volume(MPV),albumin(ALB),total bilirubin(TBIL),alanine aminotransferase(ALT),and aspartate aminotransferase(AST),in both groups were collected.The real time quantitative-polymerase chain reaction(qRT-PCR)was used to measure the expressions of circDLG1,HIF-1α,and PLGF in the two groups,and the MVD was calculated.The factors influencing PE were analyzed by the multivariate logistic regression analysis.Results The systolic and diastolic blood pressures and levels of BUN,LDH,Cr,and urinary protein in the observation group were higher than those in the control group,and the levels of MPV and ALB were lower(all P＜0.05).There were no statistical differences in age,BMI before pregnancy and at delivery,parity,gestational week,and levels of PLT,TBIL,ALT,and AST between the two groups(all P＞0.05).The results of qRT-PCR showed that the HIF-1α mRNA level in the observation group was higher than that in the control group(0.64±0.15 vs.0.47±0.11,P＜0.05),and the expression level of circDLG1 mRNA(0.62±0.24 vs.1.08±0.47),PLGF mRNA level(1.12±0.18 vs.1.68±0.23),and MVD count,(45.26±6.58 vs.75.26±8.11)were lower(all P＜0.05).The relative expression of circDLG1 in the patients was negatively correlated with HIF-1α(r=-0.564;P＜0.05),and positively with PLGF and MVD(r=0.547 and 0.621;both P＜0.05).The logistic regression analysis showed that LDH,urinary protein,and MPV were independent risk factors for PE(all P＜0.05),and the high circDLG1,PLGF,and MVD levels and a low HIF-1α level were independent protective factors for PE(all P＜0.05).Conclusions In the placental tissue of patients with PE,the expression levels of circDLG1,HIF-1α,and MVD are low,while the expression level of PLGF is high;the relative expression level of circDLG1 is closely related to HIF-1α,PLGF,and MVD;high levels of circDLG1,PLGF,and MVD and a low level of HIF-1α are independent protective factors for the occurrence of PE.关键词
子痫前期/胎盘/环状RNA DLG1/缺氧诱导因子-1α/胎盘生长因子/微血管密度Key words
Pre-eclampsia/Placenta/Circular RNA DLG1/Hypoxia-inducible factor-1α/Placental growth factor/Microvessel density引用本文复制引用
杜彩云,杨虹..子痫前期患者胎盘组织中circDLG1与HIF-1α、PLGF、MVD的表达及关系[J].国际医药卫生导报,2025,31(10):1599-1604,6.基金项目
陕西省自然科学基础研究计划(2021JM-580) Basic Research Plan of Natural Science in Shaanxi Province(2021JM-580) （2021JM-580）
